Anti-PYCARD Immunohistochemistry Kit
CAT#: VS-0325-XY1834
Our PYCARD IHC Kit features a full range of reagents, simplifying the reagent - gathering process. It is designed for easy handling, making it suitable for laboratories. Compatible with both paraffin and frozen tissue sections, it enables reliable detection of PYCARD. This is useful for studies on inflammasome activation and the role of PYCARD in mediating the assembly and activation of the inflammasome complex, which is crucial for the innate immune response to various pathogens and cellular stressors.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, nucleoli & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49074/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human spleen
Immunohistochemical staining of human spleen shows strong cytoplasmic and nuclear positivity in cells in red pulp.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15948/36925_B_7_4_selected.jpg
Normal Tissue
Figure 3 Colon
Enterocytes
Staining: Low
Intensity: Weak
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49074/136467_A_8_3.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49074/136467_A_8_8.jpg
Normal Tissue
Figure 5 Spleen
Cells in red pulp
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in white pulp
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49074/136467_B_7_4.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (MCF7, NB-4, THP-1, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000103490-PYCARD/subcellular
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Specifications
- Application
- IHC
- Size
- 50 Tests
- Species Reactivity
- Human
- Target
- PYCARD
- Primary Antibody
- Rabbit Anti-PYCARD Antibody
- Secondary Antibody
- Goat anti-Rabbit Antibody, HRP
- Sample Type
- FFPE tissue; Frozen section tissue
- Kit Storage
- All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.
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Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "PYCARD"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2948 | Hi-Affi™ Recombinant Rabbit Anti-PYCARD Monoclonal Antibody (DS2948AB) | WB, IP, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1506-CN | Recombinant Rabbit Anti-PYCARD Monoclonal Antibody (CBACN-553) | WB, IF, FC | Rabbit IgG |
| PABS-0127 | Camelid Anti-PYCARD Recombinant Single Domain Antibody (Clone VHHASC) | ELISA, Block, FuncS | Camelid VHH |
| VS3-WK1213 | Rabbit Anti-PYCARD Recombinant Antibody (VS3-WK1213) | WB, ICC, IF, FC | Rabbit IgG |
| VS7-0425-WR728 | Mouse Anti-PYCARD Recombinant Antibody (clone 1C3D3) | IHC, ICC, FC | Mouse IgG1 |
| VS7-0425-WR729 | Mouse Anti-PYCARD Recombinant Antibody (clone 1C3D7) | IHC, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC807 | AbPlus™ Anti-PYCARD Magnetic Beads (VS-0724-YC807) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5896 | Anti-Human PYCARD Immunohistochemistry Kit | IHC |
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