AbPlus™ Anti-PYCARD Magnetic Beads (VS-0724-YC807)
CAT#: VS-0724-YC807
The AbPlus Anti-PYCARD Magnetic Beads (VS-0724-YC807) is an innovative affinity resin which is bound with anti-PYCARD specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of PYCARD protein under native and denaturing conditions.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, nucleoli & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49074/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human spleen
Immunohistochemical staining of human spleen shows strong cytoplasmic and nuclear positivity in cells in red pulp.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15948/36925_B_7_4_selected.jpg
Normal Tissue
Figure 3 Colon
Enterocytes
Staining: Low
Intensity: Weak
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49074/136467_A_8_3.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49074/136467_A_8_8.jpg
Normal Tissue
Figure 5 Spleen
Cells in red pulp
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in white pulp
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49074/136467_B_7_4.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (MCF7, NB-4, THP-1, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000103490-PYCARD/subcellular
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Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-PYCARD specific antibody
- Target
- PYCARD
- Immunogen
- Recombinant human PYCARD fragment derived from E. coli.
- Target Species
- Human
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery.
- Alternative Names
- ASC; TMS; TMS1; CARD5; TMS-1
- Gene ID
- 29108
- UniProt ID
- Q9ULZ3
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "PYCARD"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2948 | Hi-Affi™ Recombinant Rabbit Anti-PYCARD Monoclonal Antibody (DS2948AB) | WB, IP, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1506-CN | Recombinant Rabbit Anti-PYCARD Monoclonal Antibody (CBACN-553) | WB, IF, FC | Rabbit IgG |
| PABS-0127 | Camelid Anti-PYCARD Recombinant Single Domain Antibody (Clone VHHASC) | ELISA, Block, FuncS | Camelid VHH |
| VS3-WK1213 | Rabbit Anti-PYCARD Recombinant Antibody (VS3-WK1213) | WB, ICC, IF, FC | Rabbit IgG |
| VS7-0425-WR728 | Mouse Anti-PYCARD Recombinant Antibody (clone 1C3D3) | IHC, ICC, FC | Mouse IgG1 |
| VS7-0425-WR729 | Mouse Anti-PYCARD Recombinant Antibody (clone 1C3D7) | IHC, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1834 | Anti-PYCARD Immunohistochemistry Kit | IHC | |
| VS-0525-XY5896 | Anti-Human PYCARD Immunohistochemistry Kit | IHC |
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