Rabbit Anti-QSOX1 Recombinant Antibody (clone R06-7A8)
CAT#: VS3-FY2073
The anti-QSOX1 antibody can be applied to western blot analysis of quiescin Q6 in A549, U2OS lysates. The recombinant monoclonal antibody exhibits selective binding to human QSOX1. It is suitable for use in a variety of assays, including WB, IP.
Tested Data
Gene Expression
WB
Figure 1 Anti-QSOX1 Antibody (VS3-FY2073) in WB.
The Quiescin Q6 expression in A549, U2OS lysates were detected using the anti-QSOX1 by western blot assay.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56243/if_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Cell line enhanced (HHSteC)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000116260-QSOX1/subcellular
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Specifications
- Immunogen
- Synthetic peptide of human Quiescin Q6
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human QSOX1
- Species Reactivity
- Human
- Clone
- R06-7A8
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 83 kDa, while the observed molecular weight stands at 83 kDa.
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Quiescin Sulfhydryl Oxidase 1; Quiescin Q6 Sulfhydryl Oxidase 1; Thiol Oxidase 1; Quiescin Q6; EC 1.8.3.2; QSCN6
- Gene ID
- 5768
- UniProt ID
- O00391
- Sequence Similarities
- Belongs to the quiescin-sulfhydryl oxidase (QSOX) family.
- Cellular Localization
- Golgi apparatus, Membrane, Secreted
- Post Translation Modifications
- N-glycosylated (PubMed:17331072, 29757379).
O-glycosylated on Thr and Ser residues (PubMed:29757379).
(O00391-QSOX1_HUMAN)
Glycosylation at Asn130, Asn243, Thr276, Thr277, Thr281, Thr282, Thr289, Ser493, Ser549, Asn575, Ser576, Thr577, Ser588, Thr590, Asn591, Thr592, Thr593, Ser604, Ser643, Thr647, and Ser655 (NX_O00391 [NX_O00391-1])
Modification sites at PhosphoSitePlus (O00391)
Glycosylation from GlyConnect QSOX1_HUMAN (1774)
Glycosylation from GlyGen (O00391) 22 sites, 30 N-linked glycans (4 sites), 3 O-linked glycans (18 sites)
- Protein Refseq
- XP_047282186.1; XP_054193878.1; NP_001004128.1; NP_002817.2
- Function
- Catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide.
Plays a role in disulfide bond formation in a variety of extracellular proteins.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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