Rabbit Anti-RABEPK Recombinant Antibody (clone R06-8K2)
CAT#: VS3-FY1811
This anti-p40 antibody detected positive in western blot analysis of p40 in K562, Hela lysates. The recombinant monoclonal antibody shows specific binding to human RABEPK. The R06-8K2 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000).
Tested Data
Gene Expression
WB
Figure 1 Anti-RABEPK Antibody (VS3-FY1811) in WB.
The p40 expression in K562, Hela lysates were detected using the anti-p40 by western blot assay.
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Subcellular Location
Figure 1 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/67106/1255_G4_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human prostate
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity with a granular pattern in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23920/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23920/53751_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23920/53751_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23920/53751_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23920/53751_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23920/53751_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23920/53751_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000136933-RABEPK
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Specifications
- Immunogen
- Recombinant human p40 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human RABEPK
- Species Reactivity
- Human
- Clone
- R06-8K2
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 41 kDa, while the observed molecular weight stands at 41 kDa.
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Rab9 Effector Protein With Kelch Motifs; 40 KDa Rab9 Effector Protein; RAB9P40; P40; Rab9 Effector P40; BA65N13.1
- Gene ID
- 10244
- UniProt ID
- Q7Z6M1
- Cellular Localization
- Cytoplasm, Endosome, Membrane
- Post Translation Modifications
- Phosphorylated on Ser residues by PIKFYVE. (Q7Z6M1-RABEK_HUMAN)
Modification sites at PhosphoSitePlus (Q7Z6M1)
Modification sites at neXtProt (NX_Q7Z6M1)
Glycosylation from GlyGen (Q7Z6M1) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_005251697.1; XP_005251698.1; XP_005251699.1; XP_005251701.1; XP_016869666.1
- Function
- Rab9 effector required for endosome to trans-Golgi network (TGN) transport.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "RABEPK"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0483MZ | Chicken Anti-RAB9P40 (ab1) Polyclonal IgY | WB | Chicken antibody |
| BRD-0484MZ | Chicken Anti-RAB9P40 (ab2) Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2969 | Hi-Affi™ Recombinant Rabbit Anti-RABEPK Monoclonal Antibody (DS2969AB) | WB, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0620F | Rabbit Anti-RABEPK Recombinant Antibody (clone ZR8) | IHC-P | Rabbit IgG |
| ZG-0440U | Rabbit Anti-RABEPK Recombinant Antibody (clone L14-A), FITC | FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1137 | AbPlus™ Anti-RABEPK Magnetic Beads (VS-0724-YC1137) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1857 | Anti-RABEPK Immunohistochemistry Kit | IHC |
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