Rabbit Anti-RAD51D Recombinant Antibody (clone R03-9E6) (CAT#: VS3-FY1866)
The R03-9E6 recombinant monoclonal antibody exhibits specific detecting to human RAD51D protein, a calculated weight of 35 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R03-9E6 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunoprecipitation (dilution range 1:20).
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RNA Expression
Figure 1 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000185379-RAD51D
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000185379-RAD51D
Specifications
- Immunogen
- Recombinant human Rad51D protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat RAD51D
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R03-9E6
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 35 kDa, while the observed molecular weight stands at 35 kDa.
- Related Disease
- Breast-Ovarian Cancer, Familial 4 and Hereditary Breast Ovarian Cancer Syndrome
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- RAD51 Paralog D; DNA Repair Protein RAD51 Homolog 4; Recombination Repair Protein; RAD51-Like Protein 3; RAD51 Homolog D; RAD51L3; R51H3
- Gene ID
- 5892
- UniProt ID
- O75771
- Sequence Similarities
- Belongs to the RecA family. RAD51 subfamily.
- Cellular Localization
- Chromosome, Cytoplasm, Cytoskeleton, Nucleus, Telomere
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (O75771)
Modification sites at neXtProt (NX_O75771)
- Protein Refseq
- NP_001136043.1; NP_002869.3; NP_598332.1
- Function
- Bind to single-stranded DNA (ssDNA) and has DNA-dependent ATPase activity. Part of the RAD51 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1760CT | Recombinant Mouse anti-Human RAD51D Monoclonal antibody (Sbe62E 6B9/5) | WB | |
| FAMAB-1239CQ | Mouse Anti-RAD51D Recombinant Antibody (clone 5A8/4) | IP, WB, ELISA | Mouse IgG1 |
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