Rabbit Anti-RANGAP1 Recombinant Antibody (clone R03-1F8)
CAT#: VS3-FY2075
Clone R03-1F8 is a rabbit recombinant monoclonal antibody that exhibits a high affinity for human RANGAP1. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. It is advised to dilute the sample for improved detectability by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20). This RanGAP1 antibody was verified by western blot analysis of RanGAP1 in 3T3 lysates and western blot analysis of RanGAP1 in Hela, A549, HL-60, U2OS, C6 lysates.
Tested Data
Gene Expression
WB
Figure 1 Anti-RANGAP1 Antibody (VS3-FY2075) in WB.
The RanGAP1 expression in 3T3 lysates were detected using the anti-RanGAP1 by western blot assay.
WB
Figure 2 Anti-RANGAP1 Antibody (VS3-FY2075) in WB.
The RanGAP1 expression in Hela, A549, HL-60, U2OS, C6 lysates were detected using the anti-RanGAP1 by western blot assay.
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nuclear membrane, cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50110/899_A8_3_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane, cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/62034/1106_H1_3_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4293/653_E2_2_selected.jpg
Normal Tissue
Figure 4 Skin
Epidermal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4293/12406_B_6_8.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4293/12149_A_8_8.jpg
Normal Tissue
Figure 6 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4293/12406_B_9_5.jpg
Normal Tissue
Figure 7 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4293/12149_A_7_3.jpg
Normal Tissue
Figure 8 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4293/12149_A_9_4.jpg
Normal Tissue
Figure 9 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4293/12149_A_9_5.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100401-RANGAP1
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Specifications
- Immunogen
- Synthetic peptide of human RanGAP1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat RANGAP1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R03-1F8
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 64 kDa, while the observed molecular weight stands at 68,82 kDa.
- Related Disease
- Parkinson Disease 7, Autosomal Recessive Early-Onset and Frontotemporal Dementia And/Or Amyotrophic Lateral Sclerosis 1
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Ran GTPase Activating Protein 1; SD; Segregation Distorter Homolog (Drosophila); Ran GTPase-Activating Protein 1; Segregation Distorter Homolog; Segregation Distortion
- Gene ID
- 5905
- UniProt ID
- P46060
- Sequence Similarities
- Belongs to the RNA1 family.
- Cellular Localization
- Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Nucleus
- Post Translation Modifications
- Phosphorylation occurs before nuclear envelope breakdown and continues throughout mitosis.
Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro.
Differential timimg of dephosphorylation occurs during phases of mitosis.
The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBE2I, on nuclear pore complex (NPC) diassembly and during mitosis.
(P46060-RAGP1_HUMAN)
Sumoylated (PubMed:11854305, 15037602, 26304119, 27160050).
Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis (PubMed:11854305).
Also required for interaction with RANBP2 and is mediated by UBE2I (PubMed:27160050).
Desumoylated by HINT1 ().
(P46060-RAGP1_HUMAN)
Ubiquitination at Lys296, Lys452, and Lys524 (NX_P46060 [NX_P46060-1])
Modification sites at PhosphoSitePlus (P46060)
Glycosylation from GlyGen (P46060) 3 sites, 1 O-linked glycan (3 sites)
- Protein Refseq
- XP_005261752.1; XP_005261753.1; XP_006724352.1; XP_011528599.1; XP_016884382.1
- Function
- GTPase activator for RAN.
Converts cytoplasmic GTP-bound RAN to GDP-bound RAN, which is essential for RAN-mediated nuclear import and export.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| MRO-1319-CN | Recombinant Rabbit Anti-RANGAP1 Monoclonal Antibody (JG37-41) | WB, IF, IHC, FC, IP | Rabbit IgG |
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| CAT | Product Name | Application | Type |
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