Rabbit Anti-RARA Recombinant Antibody (VS3-CJ560)
CAT#: VS3-CJ560
This product is a rabbit antibody that recognizes human, and mouse RARA.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58282/if_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Cell line enhanced (MCF7)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000131759-RARA
❮
❯
Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse RARA
- Species Reactivity
- Human, Mouse
- Applications
- WB, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Flow Cytometry.
Target
- Alternative Names
- RAR; NR1B1; RARalpha
- Gene ID
- 5914
- UniProt ID
- P10276
- Sequence Similarities
- Belongs to the nuclear hormone receptor family. NR1 subfamily.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylated on serine and threonine residues. Phosphorylation does not change during cell cycle. Phosphorylation on Ser-77 is crucial for transcriptional activity (By similarity).
Phosphorylation by AKT1 is required for the repressor activity but has no effect on DNA binding, protein stability nor subcellular localization. Phosphorylated by PKA in vitro. This phosphorylation on Ser-219 and Ser-369 is critical for ligand binding, nuclear localization and transcriptional activity in response to FSH signaling.
Sumoylated with SUMO2, mainly on Lys-399 which is also required for SENP6 binding. On all-trans retinoic acid (ATRA) binding, a confromational change may occur that allows sumoylation on two additional site, Lys-166 and Lys-171. Probably desumoylated by SENP6. Sumoylation levels determine nuclear localization and regulate ATRA-mediated transcriptional activity.
Trimethylation enhances heterodimerization with RXRA and positively modulates the transcriptional activation.
Ubiquitinated.
Acetylated; acetylation is increased upon pulsatile shear stress and decreased upon oscillatory shear stress.
- Protein Refseq
- NP_000955.1; NP_001019980.1; NP_001138773.1
- Function
- Receptor for retinoic acid (PubMed:19850744, PubMed:16417524, PubMed:20215566).
Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes (PubMed:28167758).
The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5 (PubMed:28167758, PubMed:19398580).
In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone deacetylation, chromatin condensation and transcriptional suppression (PubMed:16417524).
On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation (PubMed:9267036, PubMed:19850744, PubMed:20215566).
Formation of a complex with histone deacetylases might lead to inhibition of RARE DNA element binding and to transcriptional repression (PubMed:28167758).
Transcriptional activation and RARE DNA element binding might be supported by the transcription factor KLF2 (PubMed:28167758).
RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis (By similarity).
Has a role in the survival of early spermatocytes at the beginning prophase of meiosis (By similarity).
In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes (By similarity).
In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function (By similarity).
Together with RXRA, positively regulates microRNA-10a expression, thereby inhibiting the GATA6/VCAM1 signaling response to pulsatile shear stress in vascular endothelial cells (PubMed:28167758).
In association with HDAC3, HDAC5 and HDAC7 corepressors, plays a role in the repression of microRNA-10a and thereby promotes the inflammatory response (PubMed:28167758).
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-CJ560. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Related Signaling Pathways
cAMP Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "RARA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2147z | Mouse Anti-RARA Recombinant Antibody (clone 18A2) | WB, ELISA, ICC, IF, IHC | Mouse IgG2a, κ |
| MOB-1810CT | Recombinant Mouse anti-Human RARA Monoclonal antibody (3D0-2G9) | ICC, IF, IHC-P, WB, sELISA | |
| MRO-1332-CN | Recombinant Rabbit Anti-RARA Monoclonal Antibody (CBACN-482) | WB, FC | Rabbit IgG |
| MRO-2153-CN | Rabbit Anti-RARA Polyclonal Antibody (MRO-2153-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS-0625-FY1465 | Human Anti-RARA Recombinant Antibody (clone 2506W1360) | Spiked IP, IF | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3003 | Hi-Affi™ Recombinant Rabbit Anti-RARA Monoclonal Antibody (DS3003AB) | WB, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC980 | CytoStream™ Rabbit Anti-RARA Recombinant Antibody (VS13-YC980) | WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1877 | Anti-RARA Immunohistochemistry Kit | IHC | |
| VS-0525-XY5989 | Anti-Mouse RARA Immunohistochemistry Kit | IHC | |
| VS-0525-XY5988 | Anti-Human RARA Immunohistochemistry Kit | IHC | |
| VS-0525-XY5990 | Anti-Rat RARA Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: Neut, ELISA, FuncS
-2.png)
Application: WB, ELISA, FC, IHC, IP
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
