Rabbit Anti-REL Recombinant Antibody (clone R03-3D5)
CAT#: VS3-FY1988
The anti-c-Rel antibody detected in western blot analysis of c-Rel in Hela, A549, HL-60 lysates. This product is a extraordinary rabbit recombinant monoclonal antibody that is designed to compete for binding to human REL. It is suitable for use in a variety of assays, including WB, IP.
Gene Expression
Tested Data
Normal Tissue
Figure 1 IHC staining of human colon
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4404/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4404/13702_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4404/13702_A_7_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4404/13702_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4404/13702_A_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4404/13702_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (Daudi, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000162924-REL
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WB
Figure 1 Anti-REL Antibody (VS3-FY1988) in WB.
The c-Rel expression in Hela, A549, HL-60 lysates were detected using the anti-c-Rel by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human c-Rel
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human REL
- Species Reactivity
- Human
- Clone
- R03-3D5
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 69 kDa, while the observed molecular weight stands at 78 kDa.
- Related Disease
- Immunodeficiency 92 and Reticuloendotheliosis
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- REL Proto-Oncogene, NF-KB Subunit; V-Rel Avian Reticuloendotheliosis Viral Oncogene Homolog; Oncogene REL, Avian Reticuloendotheliosis; Proto-Oncogene C-Rel; C-Rel
- Gene ID
- 5966
- UniProt ID
- Q04864
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (Q04864)
Modification sites at neXtProt (NX_Q04864)
Glycosylation from GlyGen (Q04864) 8 sites, 2 O-linked glycans (8 sites)
- Protein Refseq
- XP_011531312.1; XP_016860116.1; XP_054199242.1; XP_054199243.1; NP_001278675.1
- Function
- Proto-oncogene that may play a role in differentiation and lymphopoiesis. NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "REL"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3042 | Hi-Affi™ Recombinant Rabbit Anti-REL Monoclonal Antibody (DS3042AB) | ICC, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0389-CN | Recombinant Rabbit Anti-REL Monoclonal Antibody (JM72-93) | WB, IP, IHC | Rabbit IgG |
| ZG-0436F | Mouse Anti-Rel Recombinant Antibody (ZG-0436F) | WB, IHC, IF, ELISA | Mouse IgG |
| VS3-QX967 | Mouse Anti-REL Recombinant Antibody (clone 318CT41.3.2) | WB, ICC | Mouse IgG2b |
| VS3-WK607 | Rabbit Anti-REL Recombinant Antibody (clone JM72-93) | WB, ELISA | Rabbit IgG |
| VS13-YC997 | Rabbit Anti-REL Recombinant Antibody (VS13-YC997) | WB, IHC-P, IP | Rabbit IgG |
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