Rabbit Anti-RET Recombinant Antibody (VS3-CJ479)
CAT#: VS3-CJ479
This product is a rabbit antibody that recognizes human, mouse, and rat RET.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane, cytosol & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8356/1081_D1_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human parathyroid gland
Immunohistochemical staining of human parathyroid gland shows strong positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2581/6496_A_3_8_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2581/6496_B_8_5.jpg
Normal Tissue
Figure 4 Parathyroid gland
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2581/6496_A_3_8.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2581/6496_A_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2581/6496_A_7_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (MCF7, NB-4, SH-SY5Y, THP-1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000165731-RET/subcellular
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat RET
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC, IP, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry.
Target
- Alternative Names
- PTC; MTC1; HSCR1; MEN2A; MEN2B; CDHF12; CDHR16; RET-ELE1
- Gene ID
- 5979
- UniProt ID
- P07949
- Sequence Similarities
- Belongs to the protein kinase superfamily. Tyr protein kinase family.
- Cellular Localization
- Cell membrane, Endosome, Membrane
- Post Translation Modifications
- Autophosphorylated on C-terminal tyrosine residues upon ligand stimulation.
Proteolytically cleaved by caspase-3. The soluble RET kinase fragment is able to induce cell death. The extracellular cell-membrane anchored RET cadherin fragment accelerates cell adhesion in sympathetic neurons.
- Protein Refseq
- NP_065681.1; NP_066124.1
- Function
- Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation upon binding with glial cell derived neurotrophic factor family ligands. Phosphorylates PTK2/FAK1. Regulates both cell death/survival balance and positional information. Required for the molecular mechanisms orchestration during intestine organogenesis; involved in the development of enteric nervous system and renal organogenesis during embryonic life, and promotes the formation of Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue. Modulates cell adhesion via its cleavage by caspase in sympathetic neurons and mediates cell migration in an integrin (e.g. ITGB1 and ITGB3)-dependent manner. Involved in the development of the neural crest. Active in the absence of ligand, triggering apoptosis through a mechanism that requires receptor intracellular caspase cleavage. Acts as a dependence receptor; in the presence of the ligand GDNF in somatotrophs (within pituitary), promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF. Regulates nociceptor survival and size. Triggers the differentiation of rapidly adapting (RA) mechanoreceptors. Mediator of several diseases such as neuroendocrine cancers; these diseases are characterized by aberrant integrins-regulated cell migration. Mediates, through interaction with GDF15-receptor GFRAL, GDF15-induced cell-signaling in the brainstem which induces inhibition of food-intake. Activates MAPK- and AKT-signaling pathways (PubMed:28846097, PubMed:28953886, PubMed:28846099).
Isoform 1 in complex with GFRAL induces higher activation of MAPK-signaling pathway than isoform 2 in complex with GFRAL (PubMed:28846099).
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Related Diseases
EGFR Tyrosine Kinase Inhibitor Resistance
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "RET"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-844-F(E) | Recombinant Anti-RET Antibody Fab Fragment | RIA, WB, FuncS | Fab |
| MHH-844-F(E) | Recombinant Human Anti-RET Antibody Fab Fragment | ELISA, WB, FC, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-844-S(P) | Recombinant Anti-RET Antibody scFv Fragment | FC, Neut, Funcs | scFv |
| MHH-844-S(P) | Recombinant Human Anti-RET Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-844 | Recombinant Human Anti-RET Antibody | WB, Neut, FuncS | IgG |
| ZG-065R | Mouse Anti-RET Recombinant Antibody (ZG-065R) | WB, ELISA | Mouse IgG |
| ZG-066R | Mouse Anti-RET Recombinant Antibody (ZG-066R) | WB, ELISA | Mouse IgG |
| ZG-0546U | Rabbit Anti-RET Recombinant Antibody (clone 8D12) | ELISA, FC | Rabbit IgG |
| VS7-0425-WR777 | Mouse Anti-RET Monoclonal Antibody (VS7-0425-WR777) | WB, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-066MZ | Anti-Human RET Recombinant Antibody (Y078) | Cyt, IHC, FC | Human antibody |
| TAB-066MZ-S(P) | Anti-Human RET Recombinant Antibody scFv Fragment (Y078) | IHC | Human antibody |
| TAB-066MZ-F(E) | Anti-Human RET Recombinant Antibody Fab Fragment (Y078) | IHC | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-1148MZ | Chicken Anti-Human RET Polyclonal IgY | WB, ICC | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3052 | Hi-Affi™ Recombinant Rabbit Anti-RET Monoclonal Antibody (DS3052AB) | WB, IHC, ICC, IP, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1001 | CytoStream™ Rabbit Anti-RET Recombinant Antibody (VS13-YC1001) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC312 | Recombinant Anti-RET Vesicular Antibody, EV Displayed (VS-0425-YC312) | Cyt, ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6083 | Anti-Mouse RET Immunohistochemistry Kit | IHC |
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