Mouse Anti-RHO Recombinant Antibody (clone Rho 1D4)
CAT#: FAMAB-1247CQ
This product is a recombinant mouse antibody that recognizes RHO. The antibody was purified by affinity chromatography. It can be used in IP, RIA, WB, ELISA, IF, IHC.
Published Data
IB
Figure 1 Affinity purification of CD81 receptor.
SDS-PAGE (16%) of the solubilized (Lane 1) and rho-1D4-affinity purified CD81 (Lane 2). Proteins were detected by silver staining. Lane 1, 2.5 mg of solubilized membrane protein, Lane 2, 0.5 mg of rho-1D4-purified receptor. Lanes 3 and 4 represent the immunoblot analysis of the same samples as in lanes 1 and 2, but same volume fraction of each solubilized and purified sample (1/7000) was loaded. Immunoblot analysis was done using the monoclonal antibody rho-1D4 (the epitope tag for this antibody was added to the C-terminal tail of CD81 gene).
Takayama, H., Chelikani, P., Reeves, P. J., Zhang, S., & Khorana, H. G. (2008). High-level expression, single-step immunoaffinity purification and characterization of human tetraspanin membrane protein CD81. PLoS One, 3(6), e2314.
IF
Figure 2 Localization of CD81 overexpressed in HEK293S-TetR cell line.
Mouse rho-1D4 antibody was visualized with Alexa 647 anti-mouse secondary antibody (red), overexpressed CD81-rho-1D4 was localized at the plasma membrane.
Takayama, H., Chelikani, P., Reeves, P. J., Zhang, S., & Khorana, H. G. (2008). High-level expression, single-step immunoaffinity purification and characterization of human tetraspanin membrane protein CD81. PLoS One, 3(6), e2314.
ELISA
Figure 3 Binding affinity of purified CD81 for HCV-E2.
ELISA plates were coated with rho-1D4 monoclonal antibody and purified CD81 was added, followed by the addition of increasing concentrations of HCV-E2 from 0.4 to 20 nM.
Takayama, H., Chelikani, P., Reeves, P. J., Zhang, S., & Khorana, H. G. (2008). High-level expression, single-step immunoaffinity purification and characterization of human tetraspanin membrane protein CD81. PLoS One, 3(6), e2314.
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Specifications
- Immunogen
- Bleached bovine rod outer segment (ROS)
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- C-terminus of rhodopsin
- Species Reactivity
- Bovine, Human, Zebrafish, Amphibians, Mouse, Rat
- Clone
- Rho 1D4
- Applications
- IB, IF, ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Immonobloting, Immunofluorescence and Enzyme-Linked Immunosorbent Assay.
Target
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Related Signaling Pathways
cAMP Signaling Pathway
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone Rho 1D4"
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-849-F(E) | Recombinant Anti-human RHO Antibody Fab Fragment | WB, IF, FuncS | Fab |
| MHH-849-F(E) | Recombinant Human Anti-human RHO Antibody Fab Fragment | ELISA, IF, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-849-S(P) | Recombinant Anti-human RHO Antibody scFv Fragment | RIA, ELISA, FuncS | scFv |
| MHH-849-S(P) | Recombinant Human Anti-human RHO Antibody scFv Fragment | Neut, IF, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-849 | Recombinant Human Anti-human RHO Antibody | WB, Dot, FuncS | IgG |
| MOB-2413CT | Recombinant Mouse anti-Human RHO Monoclonal antibody (FQ2626Z) | WB, IHC-P, IHC-Fr | |
| MRO-1338-CN | Recombinant Rabbit Anti-RHO Monoclonal Antibody (JA10-91) | WB, IHC | Rabbit IgG |
| FAMAB-1421CQ | Mouse Anti-RHO Recombinant Antibody (clone K42-IL) | ELISA | Mouse IgG1 |
| VS3-WK221 | Rabbit Anti-RHO Recombinant Antibody (clone JA10-91) | WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC699 | Recombinant Anti-RHO Vesicular Antibody, EV Displayed (VS-0425-YC699) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6106 | Anti-RHO Immunohistochemistry Kit | IHC | |
| VS-0525-XY6107 | Anti-Zebrafish RHO Immunohistochemistry Kit | IHC | |
| VS-0525-XY6108 | Anti-Mouse RHO Immunohistochemistry Kit | IHC | |
| VS-0525-XY6109 | Anti-Porcine RHO Immunohistochemistry Kit | IHC |
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