Mouse Anti-RHO Recombinant Antibody (clone Rho 1D4) (CAT#: FAMAB-1247CQ)

Figure 1 Affinity purification of CD81 receptor.

SDS-PAGE (16%) of the solubilized (Lane 1) and rho-1D4-affinity purified CD81 (Lane 2). Proteins were detected by silver staining. Lane 1, 2.5 mg of solubilized membrane protein, Lane 2, 0.5 mg of rho-1D4-purified receptor. Lanes 3 and 4 represent the immunoblot analysis of the same samples as in lanes 1 and 2, but same volume fraction of each solubilized and purified sample (1/7000) was loaded. Immunoblot analysis was done using the monoclonal antibody rho-1D4 (the epitope tag for this antibody was added to the C-terminal tail of CD81 gene).

Takayama, H., Chelikani, P., Reeves, P. J., Zhang, S., & Khorana, H. G. (2008). High-level expression, single-step immunoaffinity purification and characterization of human tetraspanin membrane protein CD81. PLoS One, 3(6), e2314.

Figure 2 Localization of CD81 overexpressed in HEK293S-TetR cell line.

Mouse rho-1D4 antibody was visualized with Alexa 647 anti-mouse secondary antibody (red), overexpressed CD81-rho-1D4 was localized at the plasma membrane.

Takayama, H., Chelikani, P., Reeves, P. J., Zhang, S., & Khorana, H. G. (2008). High-level expression, single-step immunoaffinity purification and characterization of human tetraspanin membrane protein CD81. PLoS One, 3(6), e2314.

Figure 3 Binding affinity of purified CD81 for HCV-E2.

ELISA plates were coated with rho-1D4 monoclonal antibody and purified CD81 was added, followed by the addition of increasing concentrations of HCV-E2 from 0.4 to 20 nM.

Takayama, H., Chelikani, P., Reeves, P. J., Zhang, S., & Khorana, H. G. (2008). High-level expression, single-step immunoaffinity purification and characterization of human tetraspanin membrane protein CD81. PLoS One, 3(6), e2314.