Anti-RHOA Immunohistochemistry Kit
CAT#: VS-0325-XY1916
Our RHOA IHC Kit features a full set of reagents, streamlining the experiment preparation. It is engineered to be user - friendly, reducing the complexity of the immunohistochemistry procedure. This kit is suitable for both paraffin - cut and frozen tissue sections, enabling reliable RHOA detection, useful for studies on cell motility, adhesion, and cytoskeletal organization, as RHOA is a small GTPase that regulates these processes.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/13314_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/13314_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/13314_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/13314_A_5_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/13314_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000067560-RHOA
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Specifications
- Application
- IHC
- Size
- 50 Tests
- Species Reactivity
- Human, Mouse, Rat
- Target
- RHOA
- Primary Antibody
- Mouse Anti-RHOA Monoclonal Antibody
- Secondary Antibody
- Goat anti-Mouse Antibody, HRP
- Sample Type
- FFPE tissue; Frozen section tissue
- Kit Storage
- All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.
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Related Signaling Pathways
Canonical Wnt Signaling Pathway
mTOR Signaling Pathway
Non-Canonical Wnt Signaling Pathway
Downloadable Resources
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Datasheet
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0510MZ | Chicken Anti-RhoA Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3067 | Hi-Affi™ Recombinant Rabbit Anti-RHOA Monoclonal Antibody (DS3067AB) | WB, IHC | IgG |
| MOR-3068 | Hi-Affi™ Recombinant Rabbit Anti-RHOA Monoclonal Antibody (DS3068AB) | ELISA, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1337-CN | Recombinant Rabbit Anti-RHOA Monoclonal Antibody (CBACN-484) | WB, IF, FC | Rabbit IgG |
| FAMAB-0306WJ | Human Anti-RHOA Recombinant Antibody (clone C1) | ELISA, WB | Human IgG |
| FAMAB-0307WJ | Human Anti-RHOA Recombinant Antibody (clone F7) | ELISA, WB | Human IgG |
| FAMAB-0308WJ | Human Anti-RHOA Recombinant Antibody (clone D10) | ELISA, WB | Human IgG |
| FAMAB-0309WJ | Human Anti-RHOA Recombinant Antibody (clone A5) | ELISA, WB | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0306WJ-S(P) | Human Anti-RHOA Recombinant Antibody (clone C1); scFv Fragment | ELISA, WB | Human scFv |
| FAMAB-0307WJ-S(P) | Human Anti-RHOA Recombinant Antibody (clone F7); scFv Fragment | ELISA, WB | Human scFv |
| FAMAB-0308WJ-S(P) | Human Anti-RHOA Recombinant Antibody (clone D10); scFv Fragment | ELISA, WB | Human scFv |
| FAMAB-0309WJ-S(P) | Human Anti-RHOA Recombinant Antibody (clone A5); scFv Fragment | ELISA, WB | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0306WJ-F(E) | Human Anti-RHOA Recombinant Antibody (clone C1); Fab Fragment | ELISA, WB | Human Fab |
| FAMAB-0307WJ-F(E) | Human Anti-RHOA Recombinant Antibody (clone F7); Fab Fragment | ELISA, WB | Human Fab |
| FAMAB-0308WJ-F(E) | Human Anti-RHOA Recombinant Antibody (clone D10); Fab Fragment | ELISA, WB | Human Fab |
| FAMAB-0309WJ-F(E) | Human Anti-RHOA Recombinant Antibody (clone A5); Fab Fragment | ELISA, WB | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC730 | AbPlus™ Anti-RHOA Magnetic Beads (VS-0724-YC730) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1003 | CytoStream™ Rabbit Anti-RHOA Recombinant Antibody (VS13-YC1003) | WB, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6112 | Anti-Mouse RHOA Immunohistochemistry Kit | IHC | |
| VS-0525-XY6111 | Anti-Human RHOA Immunohistochemistry Kit | IHC |
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