Mouse Anti-RHOA Recombinant Antibody (clone 4E12)
CAT#: VS3-WK1600
This antibody is a mouse recombinant antibody against human RHOA, which is a member of the Rho family of small GTPases. The clone 4E12 can be used in WB analysis at a dilution of 1/1000-1/2000 and IHC analysis at a dilution of 1/50-1/200.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/13314_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/13314_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/13314_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/13314_A_5_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/13314_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000067560-RHOA
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Specifications
- Immunogen
- Synthesized peptide derived from human Rho A
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human RHOA
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 4E12
- Applications
- IHC-P, WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, 0.5% BSA, 50% Glycerol
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- IHC-P: 1/50-1/200
WB: 1/1000-1/2000
Target
- Alternative Names
- Ras Homolog Family Member A; ARH12; RHO12; ARHA; Ras Homolog Gene Family, Member A; Aplysia Ras-Related Homolog 12; Small GTP Binding Protein RhoA
- Gene ID
- 387
- UniProt ID
- P61586
- Long Name
- Ras Homolog Family Member A
- Sequence Similarities
- Belongs to the small GTPase superfamily. Rho family.
- Cellular Localization
- Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Nucleus
- Post Translation Modifications
- Substrate for botulinum ADP-ribosyltransferase.
Cleaved by yopT protease when the cell is infected by some Yersinia pathogens. This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage.
- Protein Refseq
- NP_001300870.1
- Function
- Small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Mainly associated with cytoskeleton organization, in active state binds to a variety of effector proteins to regulate cellular responses such as cytoskeletal dynamics, cell migration and cell cycle.
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Related Signaling Pathways
Canonical Wnt Signaling Pathway
mTOR Signaling Pathway
Non-Canonical Wnt Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
Isotype Control
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| FAMAB-0306WJ-S(P) | Human Anti-RHOA Recombinant Antibody (clone C1); scFv Fragment | ELISA, WB | Human scFv |
| FAMAB-0307WJ-S(P) | Human Anti-RHOA Recombinant Antibody (clone F7); scFv Fragment | ELISA, WB | Human scFv |
| FAMAB-0308WJ-S(P) | Human Anti-RHOA Recombinant Antibody (clone D10); scFv Fragment | ELISA, WB | Human scFv |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0306WJ-F(E) | Human Anti-RHOA Recombinant Antibody (clone C1); Fab Fragment | ELISA, WB | Human Fab |
| FAMAB-0307WJ-F(E) | Human Anti-RHOA Recombinant Antibody (clone F7); Fab Fragment | ELISA, WB | Human Fab |
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| CAT | Product Name | Application | Type |
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