Rabbit Anti-RHOA Recombinant Antibody (clone R58-4S-7)
CAT#: VS3-FY2110
This R58-4S-7 recombinant monoclonal antibody exhibits a high affinity for human RHOA protein, a calculated weight of 22 kDa. It cross-reacts with mouse, rat. The recommend antibody dilution is determined as western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/100-1/200), immunofluorescence (dilution 1/50-1/200), flow cytometry (dilution 1/50).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/13314_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/13314_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/13314_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/13314_A_5_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5052/13314_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000067560-RHOA
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat RHOA
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R58-4S-7
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence, Flow Cytometry
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 22 kDa, while the observed molecular weight stands at 22kDa.
- Related Disease
- Ectodermal Dysplasia With Facial Dysmorphism And Acral, Ocular, And Brain Anomalies and Linear Hypopigmentation And Craniofacial Asymmetry With Acral, Ocular And Brain Anomalies
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Immunofluorescence: 1/50-1/200
Flow Cytometry: 1/50
Target
- Alternative Names
- Ras Homolog Family Member A; ARH12; RHO12; ARHA; Ras Homolog Gene Family, Member A; Aplysia Ras-Related Homolog 12; Small GTP Binding Protein RhoA
- Gene ID
- 387
- UniProt ID
- P61586
- Sequence Similarities
- Belongs to the small GTPase superfamily. Rho family.
- Cellular Localization
- Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Nucleus
- Post Translation Modifications
- (Microbial infection) Substrate for botulinum ADP-ribosyltransferase. (P61586-RHOA_HUMAN)
(Microbial infection) Cleaved by yopT protease when the cell is infected by some Yersinia pathogens.
This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage.
(P61586-RHOA_HUMAN)
(Microbial infection) AMPylation at Tyr-34 and Thr-37 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively.
AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells.
- Protein Refseq
- NP_001300870.1; NP_001300872.1; NP_001300873.1; NP_001300874.1; NP_001300875.1
- Function
- Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers.
Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis.
Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion.
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Related Signaling Pathways
Canonical Wnt Signaling Pathway
mTOR Signaling Pathway
Non-Canonical Wnt Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0510MZ | Chicken Anti-RhoA Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
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| MOR-3067 | Hi-Affi™ Recombinant Rabbit Anti-RHOA Monoclonal Antibody (DS3067AB) | WB, IHC | IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0306WJ | Human Anti-RHOA Recombinant Antibody (clone C1) | ELISA, WB | Human IgG |
| FAMAB-0307WJ | Human Anti-RHOA Recombinant Antibody (clone F7) | ELISA, WB | Human IgG |
| FAMAB-0308WJ | Human Anti-RHOA Recombinant Antibody (clone D10) | ELISA, WB | Human IgG |
| FAMAB-0309WJ | Human Anti-RHOA Recombinant Antibody (clone A5) | ELISA, WB | Human IgG |
| VS7-0425-WR780 | Mouse Anti-RHOA Recombinant Antibody (clone 7E7H4) | WB, IHC, ICC, FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0306WJ-S(P) | Human Anti-RHOA Recombinant Antibody (clone C1); scFv Fragment | ELISA, WB | Human scFv |
| FAMAB-0307WJ-S(P) | Human Anti-RHOA Recombinant Antibody (clone F7); scFv Fragment | ELISA, WB | Human scFv |
| FAMAB-0308WJ-S(P) | Human Anti-RHOA Recombinant Antibody (clone D10); scFv Fragment | ELISA, WB | Human scFv |
| FAMAB-0309WJ-S(P) | Human Anti-RHOA Recombinant Antibody (clone A5); scFv Fragment | ELISA, WB | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0306WJ-F(E) | Human Anti-RHOA Recombinant Antibody (clone C1); Fab Fragment | ELISA, WB | Human Fab |
| FAMAB-0307WJ-F(E) | Human Anti-RHOA Recombinant Antibody (clone F7); Fab Fragment | ELISA, WB | Human Fab |
| FAMAB-0308WJ-F(E) | Human Anti-RHOA Recombinant Antibody (clone D10); Fab Fragment | ELISA, WB | Human Fab |
| FAMAB-0309WJ-F(E) | Human Anti-RHOA Recombinant Antibody (clone A5); Fab Fragment | ELISA, WB | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC730 | AbPlus™ Anti-RHOA Magnetic Beads (VS-0724-YC730) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1003 | CytoStream™ Rabbit Anti-RHOA Recombinant Antibody (VS13-YC1003) | WB, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1916 | Anti-RHOA Immunohistochemistry Kit | IHC | |
| VS-0525-XY6112 | Anti-Mouse RHOA Immunohistochemistry Kit | IHC | |
| VS-0525-XY6111 | Anti-Human RHOA Immunohistochemistry Kit | IHC |
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