Rabbit Anti-RHOA Recombinant Antibody (clone R58-4S-7) (CAT#: VS3-FY2110)

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse and Rat RHOA

• Species Reactivity
• Human, Mouse, Rat

• Clone
• R58-4S-7

• Applications
• Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence, Flow Cytometry

• Conjugate
• Unconjugated

• MW
• The calculated weight of the target protein is approximately 22 kDa, while the observed molecular weight stands at 22kDa.

• Related Disease
• Ectodermal Dysplasia With Facial Dysmorphism And Acral, Ocular, And Brain Anomalies and Linear Hypopigmentation And Craniofacial Asymmetry With Acral, Ocular And Brain Anomalies

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• PBS (pH 7.4), 150 mM NaCl, 50% glycerol

• Preservative
• 0.02% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunohistochemistry: 1/100-1/200
  Immunofluorescence: 1/50-1/200
  Flow Cytometry: 1/50

• Alternative Names
• Ras Homolog Family Member A; ARH12; RHO12; ARHA; Ras Homolog Gene Family, Member A; Aplysia Ras-Related Homolog 12; Small GTP Binding Protein RhoA

• Gene ID
• 387

• UniProt ID
• P61586

• Sequence Similarities
• Belongs to the small GTPase superfamily. Rho family.

• Cellular Localization
• Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Nucleus

• Post Translation Modifications
• (Microbial infection) Substrate for botulinum ADP-ribosyltransferase. (P61586-RHOA_HUMAN)
  (Microbial infection) Cleaved by yopT protease when the cell is infected by some Yersinia pathogens.
  This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage.
  (P61586-RHOA_HUMAN)
  (Microbial infection) AMPylation at Tyr-34 and Thr-37 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively.
  AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells.

• Protein Refseq
• NP_001300870.1; NP_001300872.1; NP_001300873.1; NP_001300874.1; NP_001300875.1

• Function
• Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers.
  Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis.
  Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

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