Rat Anti-RNA pol II CTD phospho Ser2 Recombinant Antibody (clone 0725W11)
CAT#: VS-0725-FY166
This recombinant antibody, clone 0725W11, targets the RNA Pol II CTD sequence specifically phosphorylated at serine 2, with the immunogen being a peptide containing this modification. It is validated for applications such as Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Sequencing (ChIP-Seq), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), and Western Blot (WB). RNA polymerase II, responsible for mRNA synthesis, relies on its carboxy-terminal domain (CTD) for essential functions. This antibody provides a valuable resource for investigating RNA Pol II activity and gene expression.
Specifications
- Immunogen
- A peptide containing the RNA Pol II CTD sequence, phosphorylated at serine 2.
- Host Species
- Rat
- Type
- Rat IgG1
- Specificity
- Human RNA pol II CTD phospho Ser2
- Species Reactivity
- Human, Mouse
- Clone
- 0725W11
- Applications
- Chromatin Immunoprecipitation, Chromatin Immunoprecipitation-Sequencing, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Product Property
- Purification
- Protein G purified
- Molecular Weight
- The molecular weight of the antigen is 240 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol
- Preservative
- 0.035% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 0.5-2 µg/mL
Chromatin Immunoprecipitation: 20 µg per Chromatin Immunoprecipitation
Chromatin Immunoprecipitation-Sequencing: 20 µg each
Immunofluorescence: 1:500
Target
- Alternative Names
- RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220
- Long Name
- RNA polymerase II C-terminal domain phosphorylated at Serine 2
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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