Anti-RNA pol II CTD phospho Ser5 Recombinant Antibody (VS-0725-FY165)
CAT#: VS-0725-FY165
This is a recombinant antibody raised against a peptide containing the RNA Pol II CTD sequence, specifically phosphorylated at serine 5. It is suitable for Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Sequencing (ChIP-Seq), Immunocytochemistry (ICC), Immunofluorescence (IF), and Western Blot (WB). RNA polymerase II is the primary enzyme responsible for mRNA synthesis in eukaryotes, with its CTD repeats essential for activity. This antibody is an important tool for studying transcriptional processes.
| Sub Cat | Host Animal | Application | Clone | Size | Conjugation | Endotoxin | Purity | Fc Engineering | |
| VS-0725-FY165-1 | Rat | ChIP, ChIP-Seq, ICC, IF, IP, WB | 0725W10 | ||||||
| VS-0725-FY165-2 | Mouse | ChIP, ChIP-Seq, ELISA, ICC, IF, WB | 0725W74 |
Specifications
- Immunogen
- A peptide containing the RNA Pol II CTD sequence, specifically phosphorylated at serine 5.
- Specificity
- Human RNA pol II CTD phospho Ser5
- Species Reactivity
- Human
- Related Disease
- Breast Cancer
Product Property
- Molecular Weight
- The molecular weight of the antigen is 240 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol
- Preservative
- 0.035% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Chromatin Immunoprecipitation: 5-10 µg per Chromatin Immunoprecipitation
Chromatin Immunoprecipitation-Sequencing: 5-10 µg each
Immunocytochemistry/Immunofluorescence: 1-2 µg/mL
Western Blot: 0.5-2 µg/mL
Target
- Alternative Names
- RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220
- Long Name
- RNA polymerase II C-terminal domain phosphorylated at Serine 5
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-0725-FY165. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See Our Custom Production in Action
Popular Products
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: Block, Cyt, FuncS, Inhib
Application: WB, IF, FuncS
Application: ELISA
Application: WB, ELISA, FuncS, IB, FC, SPR, Apop
Application: WB, ELISA, FC, IHC, IF, IP
Application: ELISA, FC, Neut, Inhib
-2.png)
Application: ELISA, WB
-2.png)
Application: ELISA
Application: IF, ICC, WB, IHC-P, IP
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
