Mouse Anti-RNA pol II CTD Ser2ph/Ser5ph Recombinant Antibody (clone 0725W16)
CAT#: VS-0725-FY171
The Mouse Anti-RNA pol II CTD Ser2ph/Ser5ph Recombinant Antibody (clone 0725W16) is a recombinant antibody targeting both phospho-serine 2 and phospho-serine 5 within the CTD of human RNA Polymerase II, with a synthetic peptide featuring these modifications as its immunogen. It is validated for ChIP, ChIP-Seq, Immunocytochemistry (ICC), Immunofluorescence (IF), and Western Blot (WB). RNA polymerase II's CTD phosphorylation is crucial for its activity and gene transcription, making this antibody an important tool for studying these complex regulatory mechanisms.
Specifications
- Immunogen
- A synthetic peptide featuring both phospho-serine 2 and phospho-serine 5 within the CTD of human RNA Polymerase II.
- Host Species
- Mouse
- Type
- Mouse IgG1, kappa
- Specificity
- Human RNA pol II CTD Ser2ph/Ser5ph
- Species Reactivity
- Human
- Clone
- 0725W16
- Applications
- Chromatin Immunoprecipitation, Chromatin Immunoprecipitation-Sequencing, Immunocytochemistry, Immunofluorescence, Western Blot
- Related Disease
- Cancer, Neurodegenerative diseases, Viral infections
Product Property
- Purification
- Protein A purified
- Molecular Weight
- The molecular weight of the antigen is 240 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS, 30% glycerol
- Preservative
- 0.035% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Chromatin Immunoprecipitation: 5-10 µg per Chromatin Immunoprecipitation
Chromatin Immunoprecipitation-Sequencing: 5-10 µg each
Immunocytochemistry/Immunofluorescence: 5 µg/mL
Western Blot: 0.5-2 µg/mL
Target
- Alternative Names
- RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220
- Long Name
- RNA polymerase II C-terminal domain Serine 2 phosphorylation/Serine 5 phosphorylation (referring to dual phosphorylation)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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