Mouse Anti-RNA pol II Recombinant Antibody (clone 4H8)
CAT#: VS-0725-FY172
This recombinant antibody, clone 4H8, targets a synthetic peptide (YSPTSpPS) corresponding to human RNA Pol II, capable of recognizing both phosphorylated and unphosphorylated forms of the largest subunit. It is suitable for Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Sequencing (ChIP-Seq), Immunocytochemistry (ICC), Immunofluorescence (IF), and Western Blot (WB). RNA polymerase II is the primary enzyme in eukaryotes for mRNA synthesis, with its C-terminal domain (CTD) essential for activity. This antibody is a versatile tool for studying gene expression and transcriptional processes.
Specifications
- Immunogen
- A synthetic peptide (YSPTSpPS) corresponding to human RNA Pol II. It is capable of recognizing both the phosphorylated and unphosphorylated forms of the largest subunit of RNA Pol II.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Budding Yeast RNA pol II
- Species Reactivity
- Budding Yeast, C. elegans, Human, Mouse, Rat
- Clone
- 4H8
- Applications
- Chromatin Immunoprecipitation, Chromatin Immunoprecipitation-Sequencing, Immunocytochemistry, Immunofluorescence, Western Blot
- Related Disease
- Cancer, Neurodegenerative diseases, Viral infections
Product Property
- Purification
- Affinity purified
- Molecular Weight
- The molecular weight of the antigen is 217 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS, 0.2% gelatin, 50% glycerol
- Preservative
- 0.1% Sodium azide, 0.01% thimerosal
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Chromatin Immunoprecipitation: 10 µL per Chromatin Immunoprecipitation
Chromatin Immunoprecipitation-Sequencing: 6-20 µL each
Western Blot: 1:2000-1:5000
Target
- Alternative Names
- RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220
- Long Name
- RNA Polymerase II
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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