Rabbit Anti-RNF20 Recombinant Antibody (clone 2H12) (CAT#: ZG-0098U)
The purified Rabbit Anti-RNF20 Recombinant Antibody (clone 2H12) displayed high specificity against the RNF20 protein of human.
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WB
Figure 1 Rabbit Anti-RNF20 Antibody (ZG-0098U) in WB
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate. All lanes: RNF20 antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 114 kDa
Observed band size: 114 kDa
IHC
Figure 2 Rabbit Anti-RNF20 Antibody (ZG-0098U) in IHC
IHC image of ZG-0098U diluted at 1:100 and staining in paraffin-embedded human melanoma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IF
Figure 3 Rabbit Anti-RNF20 Antibody (ZG-0098U) in IF
Immunofluorescence staining of Hela Cells with ZG-0098U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human RNF20
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human RNF20
- Species Reactivity
- Human
- Clone
- 2H12
- Applications
- ELISA, WB, IHC, IF
- Conjugate
- Unconjugated
- Related Disease
- Inflammation, Inflammation-Associated Cancer and inflammatory bowel disease
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot, Immunohistochemistry, Immunofluorescence. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200
Target
- Alternative Names
- E3 ubiquitin-protein ligase BRE1A; BRE1-A; hBRE1; EC 2.3.2.27; RING finger protein 20; RING-type E3 ubiquitin transferase BRE1A; RNF20; BRE1A
- Gene ID
- 56254
- UniProt ID
- Q5VTR2
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "RNF20"
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IgG Antibodies
Rabbit Monoclonal Antibodies
IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1045CT | Recombinant Mouse anti-Human RNF20 Monoclonal antibody (SOG30-96) | I-ELISA, ICC, IF, WB | |
| MRO-1341-CN | Recombinant Rabbit Anti-RNF20 Monoclonal Antibody (CBACN-486) | WB, IP, FC | Rabbit IgG |
| VS3-WK1029 | Mouse Anti-RNF20 Recombinant Antibody (VS3-WK1029) | WB | Mouse IgG |
| VS3-WK1030 | Rabbit Anti-RNF20 Recombinant Antibody (VS3-WK1030) | WB, IP, FC | Rabbit IgG |
| VS3-XY3487 | Rabbit Anti-RNF20 Recombinant Antibody (clone R09-2F6) | WB, ICC, IF, IP, FC | Rabbit IgG |
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