Rabbit Anti-RPA2 (pT21) Recombinant Antibody (clone R02-2I5)

Name: Rabbit Anti-RPA2 (pT21) Recombinant Antibody (clone R02-2I5)
SKU: VS3-WK2133
Rating: 5 (1 reviews)

CAT#: VS3-WK2133

This product is a rabbit recombinant antibody that is specific to phosphorylated RPA2. The target of this antibody is phospho-RPA2 (Thr21). The clone R02-2I5 can be used in WB analysis at a dilution of 1/500-1/1000 and IP analysis at a dilution of 1/20. The molecular weight of RPA2 (pThr21) observed in WB experiments using this antibody is 32 kDa.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26306/220_B6_1_selected.jpg

Normal Tissue Figure 2 Kidney

Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_A_8_5.jpg

Normal Tissue Figure 3 Testis

Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_A_6_6.jpg

Normal Tissue Figure 4 Skin

Cells in basal layer Staining: Medium Intensity: Moderate Quantity:>75% Cells in granular layer Staining: Medium Intensity: Moderate Quantity: 75%-25% Cells in spinous layer Staining: Medium Intensity: Moderate Quantity:>75% Endothelial cells Staining: Medium Intensity: Moderate Fibrohistiocytic cells Staining: High Intensity: Strong Langerhans cells Staining: Medium Intensity: Moderate Quantity:>75% Lymphocytes Staining: High Intensity: Strong Melanocytes Staining: High Intensity: Strong Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_B_7_1.jpg

Normal Tissue Figure 5 Lymph node

Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_A_9_8.jpg

Normal Tissue Figure 6 Colon

Endocrine cells Staining: High Intensity: Strong Endothelial cells Staining: High Intensity: Strong Quantity:>75% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Fibroblasts Staining: High Intensity: Strong Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: High Intensity: Strong

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_A_9_3.jpg

Normal Tissue Figure 7 Liver

Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_A_8_4.jpg

RNA Expression Figure 8 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000117748-RPA2

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Specifications

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Phospho-Thr21 of human, mouse and rat RPA2

Species Reactivity
Human, Mouse, Rat

Clone
R02-2I5

Applications
WB, IP

Conjugate
Unconjugated

Product Property

Clonality
Monoclonal

Purification
Protein A affinity purified

Purity
>95% as determined by SDS-PAGE

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS (pH 7.4), 150 mM NaCl and 50% Glycerol

Preservative
0.02% Sodium azide

Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping
Ice packs

Applications

Application Notes
WB: 1/500-1/1000
IP: 1/20

Target

Alternative Names
Replication Protein A2; Replication Protein A 34 KDa Subunit; Replication Factor A Protein 2; RF-A Protein 2; RP-A P32; RP-A P34; REPA2

Gene ID
6118

UniProt ID
P15927

Long Name
Replication Protein A2

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS3-WK2133.pdf

MSDS

MSDS - VS3-WK2133.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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