Rabbit Anti-RPA2 Recombinant Antibody (clone R07-4D1)
CAT#: VS3-FY1872
The R07-4D1 recombinant monoclonal antibody shows a high affinity for human RPA2 protein, a calculated weight of 29 kDa. It cross-reacts with mouse, rat, hamster.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R07-4D1 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:50-1:100), immunoprecipitation (dilution range 1:20).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26306/220_B6_1_selected.jpg
Normal Tissue
Figure 2 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_A_8_5.jpg
Normal Tissue
Figure 3 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_A_6_6.jpg
Normal Tissue
Figure 4 Skin
Cells in basal layer Staining: Medium Intensity: Moderate Quantity:>75% Cells in granular layer Staining: Medium Intensity: Moderate Quantity: 75%-25% Cells in spinous layer Staining: Medium Intensity: Moderate Quantity:>75% Endothelial cells Staining: Medium Intensity: Moderate Fibrohistiocytic cells Staining: High Intensity: Strong Langerhans cells Staining: Medium Intensity: Moderate Quantity:>75% Lymphocytes Staining: High Intensity: Strong Melanocytes Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_B_7_1.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_A_9_8.jpg
Normal Tissue
Figure 6 Colon
Endocrine cells Staining: High Intensity: Strong Endothelial cells Staining: High Intensity: Strong Quantity:>75% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Fibroblasts Staining: High Intensity: Strong Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_A_8_4.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000117748-RPA2
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Specifications
- Immunogen
- Synthetic peptides of human RPA32/RPA2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat, Hamster RPA2
- Species Reactivity
- Human, Mouse, Rat, Hamster
- Clone
- R07-4D1
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 29 kDa, while the observed molecular weight stands at 29 kDa.
- Related Disease
- Ataxia-Telangiectasia and Xeroderma Pigmentosum, Variant Type
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Replication Protein A2; Replication Protein A 34 KDa Subunit; Replication Factor A Protein 2; RF-A Protein 2; RP-A P32; RP-A P34; REPA2
- Gene ID
- 6118
- UniProt ID
- P15927
- Sequence Similarities
- Belongs to the replication factor A protein 2 family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Differentially phosphorylated throughout the cell cycle, becoming phosphorylated at the G1-S transition and dephosphorylated in late mitosis.
Mainly phosphorylated at Ser-23 and Ser-29, by cyclin A-CDK2 and cyclin B-CDK1, respectively during DNA replication and mitosis.
Dephosphorylation may require the serine/threonine-protein phosphatase 4.
Phosphorylation at Ser-23 and Ser-29 is a prerequisite for further phosphorylation.
Becomes hyperphosphorylated on additional residues including Ser-4, Ser-8, Thr-21 and Ser-33 in response to DNA damage.
Hyperphosphorylation is mediated by ATM, ATR and PRKDC.
Primarily recruited to DNA repair nuclear foci as a hypophosphorylated form it undergoes subsequent hyperphosphorylation, catalyzed by ATR.
Hyperphosphorylation is required for RAD51 recruitment to chromatin and efficient DNA repair.
Phosphorylation at Thr-21 depends upon RFWD3 presence.
- Protein Refseq
- XP_024304630.1; XP_024304632.1; XP_054194063.1; XP_054194064.1; NP_001273005.1
- Function
- As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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