Rabbit Anti-RPL13 Recombinant Antibody (clone R14-3X-9)
CAT#: VS3-FY2174
Clone R14-3X-9 is a rabbit recombinant antibody that shows a high affinity for human RPL13 protein, a calculated weight of 24 kDa. It cross-reacts with mouse.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R14-3X-9 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:100-1:200), immunofluorescence (dilution range 1:50-1:200).
Gene Expression
Normal Tissue
Figure 1 IHC staining of human esophagus
Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51702/118817_B_5_1_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51702/118817_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51702/118817_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51702/118817_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51702/118817_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51702/118817_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51702/118817_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000167526-RPL13
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse RPL13
- Species Reactivity
- Human, Mouse
- Clone
- R14-3X-9
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 24 kDa, while the observed molecular weight stands at 26kDa.
- Related Disease
- Spondyloepimetaphyseal Dysplasia, Isidor-Toutain Type and Spondyloepimetaphyseal Dysplasia
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Ribosomal Protein L13; Large Ribosomal Subunit Protein EL13; Breast Basic Conserved Protein 1; BBC1; 0S Ribosomal Protein L13; OK/SW-Cl.46; D16S444E; D16S44E; L13
- Gene ID
- 6137
- UniProt ID
- P26373
- Sequence Similarities
- Belongs to the eukaryotic ribosomal protein eL13 family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Ubiquitination at Lys145, Lys105, Lys123, Lys174, and Lys177 (NX_P26373 [NX_P26373-1])
Modification sites at PhosphoSitePlus (P26373)
Glycosylation from GlyGen (P26373) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_000968.2; NP_001230060.1; NP_150254.1
- Function
- The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules (Probable). The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain (Probable).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "RPL13"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0799MZ | Recombinant Mouse Anti-Human RPL13 Antibody | ICC, IF | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3108 | Hi-Affi™ Recombinant Rabbit Anti-RPL13 Monoclonal Antibody (DS3108AB) | WB, IHC, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC4381 | PE-A*03:01/Human RPL13 (ARLFGIRAK) MHC Tetramer | FCM | |
| MHC-LC4382 | PE-A*11:01/Human RPL13 (ARLFGIRAK) MHC Tetramer | FCM | |
| MHC-LC4383 | PE-A*31:01/Human RPL13 (ARLFGIRAK) MHC Tetramer | FCM | |
| MHC-LC4384 | PE-B*27:05/Human RPL13 (ARLFGIRAK) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC737 | AbPlus™ Anti-RPL13 Magnetic Beads (VS-0724-YC737) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1943 | Anti-RPL13 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6191 | Anti-Human RPL13 Immunohistochemistry Kit | IHC |
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