Mouse Anti-RPS27 Monoclonal Antibody (clone 4A12)
CAT#: VS7-0425-WR410
This product is a mouse IgG1 antibody that binds to human RPS27. The antibody 4A12 can be used for IHC assay.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli, cytosol & endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/66851/1270_H8_3_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000177954-RPS27
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Specifications
- Immunogen
- Recombinant human RPS27 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human RPS27
- Species Reactivity
- Human
- Clone
- 4A12
- Molecule Class
- Serine/Threonine Protein Kinase
- Applications
- IHC
- Conjugate
- Unconjugated
- Related Disease
- Diamond-Blackfan anemia 17 (DBA17)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Immunohistochemistry-Paraffin: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- S27; RPS27; eS27; DBA17; MPS-1
- Gene ID
- 6232
- UniProt ID
- P42677
- Long Name
- Ribosomal Protein S27
- Cellular Localization
- Cytoplasm; Nucleus
- Post Translation Modifications
- Phosphoprotein
- Protein Refseq
- NP_001021.1
- Function
- Ribonucleoprotein; Ribosomal protein
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "Clone 4A12"
- CAT
- Product Name
See other products for "RPS27"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-067R | Mouse Anti-RPS27 Recombinant Antibody (ZG-067R) | IF, ELISA | Mouse IgG |
| ZG-068R | Mouse Anti-RPS27 Recombinant Antibody (ZG-068R) | IF, ELISA | Mouse IgG |
| VS7-0425-WR411 | Mouse Anti-RPS27 Monoclonal Antibody (clone 7E3) | ICC | Mouse IgG1 |
| VS7-0425-WR792 | Mouse Anti-RPS27 Monoclonal Antibody (VS7-0425-WR792) | IHC-P, IF, ELISA | Mouse IgG |
| VS7-0425-WR793 | Mouse Anti-RPS27 Monoclonal Antibody (VS7-0425-WR793) | IF, ICC, ELISA | Mouse IgG |
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