Rabbit Anti-RPS6KA1 (pS380) Recombinant Antibody (clone R03-8I3)
CAT#: VS3-WK2122
This product is a rabbit recombinant antibody that is specific to phosphorylated RPS6KA1. The target of this antibody is phospho-RPS6KA1 (Ser380). The clone R03-8I3 can be used in WB analysis at a dilution of 1/500-1/1000, IF analysis at a dilution of 1/50-1/200 and IP analysis at a dilution of 1/20. The molecular weight of RPS6KA1 (pSer380) observed in WB experiments using this antibody is 90 kDa.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/21_H4_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/23318_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/23318_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/23318_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/23318_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Preleptotene spermatocytes
Staining: Low
Intensity: Moderate
Quantity: <25%
Round or early spermatids
Staining: Low
Intensity: Moderate
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/23318_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7981/23318_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (HMC-1, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000117676-RPS6KA1/subcellular
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Ser380 of human, mouse and rat RPS6KA1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R03-8I3
- Applications
- WB, ICC, IF, IP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
IP: 1/20
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
See other products for "RPS6KA1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0827MZ | Recombinant Mouse Anti-Human RPS6KA1 Antibody (clone 8C12) | ELISA | Mouse antibody |
| VS3-QX981 | Mouse Anti-RPS6KA1 Recombinant Antibody (clone 6B9-D6-F8) | WB, IP | Mouse IgG1 |
| VS3-QX982 | Mouse Anti-RPS6KA1 Recombinant Antibody (clone 205CT18.3.1) | WB | Mouse IgG1, κ |
| VS3-WK2045 | Rabbit Anti-RPS6KA1 (pS380) Recombinant Antibody (clone R05-7F3) | WB, IP | Rabbit IgG |
| VS3-WK2050 | Rabbit Anti-RPS6KA1 (pT359/pS363) Recombinant Antibody (clone R33-5H-9) | WB, IHC-P, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3121 | Hi-Affi™ Recombinant Rabbit Anti-RPS6KA1 Monoclonal Antibody (DS3121AB) | ICC, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY227 | AbPlus™ Anti-RPS6KA1 Magnetic Beads (CBACN-658) | IP, Protein Purification | |
| VS-0724-YC1347 | AbPlus™ Anti-RPS6KA1 Magnetic Beads (VS-0724-YC1347) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1018 | CytoStream™ Rabbit Anti-RPS6KA1 Recombinant Antibody (VS13-YC1018) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6219 | Anti-RPS6KA1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6220 | Anti-Mouse RPS6KA1 Immunohistochemistry Kit | IHC |
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