Rabbit Anti-RPS6KA3 (pS227) Recombinant Antibody (VS3-WK2265) (CAT#: VS3-WK2265)
This product is a rabbit recombinant antibody that is specific to phosphorylated RPS6KA3. The target of this antibody is phospho-RPS6KA3 (Ser227). It can be used in IP analysis at a dilution of 1/20 and WB analysis at a dilution of 1/500-1/1000.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, nucleoli & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3221/91_C11_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, nucleoli & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3221/91_C11_2_selected.jpg
Figure 2 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3221/9882_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3221/9882_A_8_8.jpg
Figure 3 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3221/9882_B_8_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3221/9882_B_8_5.jpg
Figure 4 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3221/9882_A_8_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3221/9882_A_8_3.jpg
Figure 5 Liver
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3221/9882_A_9_4.jpg
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3221/9882_A_9_4.jpg
Figure 6 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3221/9882_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3221/9882_A_8_5.jpg
Figure 7 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3221/9882_A_6_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3221/9882_A_6_6.jpg
Figure 8 RNA cell line category: Cell line enhanced (Hep G2)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000177189-RPS6KA3
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000177189-RPS6KA3
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Ser227 of human, mouse and rat RPS6KA3
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- IP: 1/20
WB: 1/500-1/1000
Target
- Alternative Names
- RPS6KA3; ISPK1; MAPKAPK1B; RSK2; Ribosomal protein S6 kinase alpha-3; S6K-alpha-3; 90 kDa ribosomal protein S6 kinase 3; p90-RSK 3; p90RSK3; Insulin-stimulated protein kinase 1; ISPK-1; MAP kinase-activated protein kinase 1b; MAPK-activated
- Gene ID
- 6197
- UniProt ID
- P51812
- Long Name
- Ribosomal Protein S6 Kinase A3
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3927z | Mouse Anti-RPS6KA3 Recombinant Antibody (clone 18G7) | WB, ELISA, FC, IF | Mouse IgG1 |
| VS3-QX984 | Mouse Anti-RPS6KA3 Recombinant Antibody (clone 5H5-C11-F10) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-XY1386 | Mouse Anti-RPS6KA3 Recombinant Antibody (clone 356CT10.6.1.2) | WB | Mouse IgG1 |
| VS3-FY1282 | Recombinant Rabbit Anti-RPS6KA3 (phospho Ser227) Antibody (clone R01-5B5) | WB, IP | Rabbit IgG |
| VS3-FY1283 | Recombinant Rabbit Anti-RPS6KA3 Antibody (clone R05-6E3) | WB, IHC-P, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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