Rabbit Anti-RPS6KB1 (pT421/pS424) Recombinant Antibody (clone R09-3B7) (CAT#: VS3-WK2109)
This product is a rabbit recombinant antibody that is specific to phosphorylated RPS6KB1. The target of this antibody is phospho-RPS6KB1 (Thr421/Ser424). The clone R09-3B7 can be used in WB analysis at a dilution of 1/500-1/1000 and IP analysis at a dilution of 1/20.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39442/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39442/if_selected.jpg
Figure 2 IHC staining of human bone marrow
(Immunohistochemical staining of human bone marrow shows distinct nuclear positivity in hematopoietic cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39442/ihc_selected.jpg
(Immunohistochemical staining of human bone marrow shows distinct nuclear positivity in hematopoietic cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39442/ihc_selected.jpg
Figure 3 IHC staining of human cerebellum
(Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3838/ihc_selected.jpg
(Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3838/ihc_selected.jpg
Figure 4 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/ihc_selected.jpg
(Immunohistochemical staining of human stomach shows cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/ihc_selected.jpg
Figure 5 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/42029_A_9_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/42029_A_9_8.jpg
Figure 6 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/42029_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/42029_A_8_5.jpg
Figure 7 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/42029_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/42029_A_6_6.jpg
Figure 8 RNA cell line category: Cell line enriched (MCF7)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000108443-RPS6KB1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000108443-RPS6KB1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Thr421/Ser424 of human and rat RPS6KB1
- Species Reactivity
- Human, Rat
- Clone
- R09-3B7
- Applications
- WB, IP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IP: 1/20
Target
- Alternative Names
- Ribosomal Protein S6 Kinase B1; Serine/Threonine-Protein Kinase 14A; Ribosomal Protein S6 Kinase I; EC 2.7.11.1; S6K-Beta-1; P70 S6KA; STK14A; S6K1; Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1; Ribosomal Protein S6 Kinase, 70kD, Polypeptide 1; 70 KDa Ribosomal Protein S6 Kinase 1; Ribosomal Protein S6 Kinase Beta-1; Serine/Threonine Kinase 14 Alpha
- Gene ID
- 6198
- UniProt ID
- P23443
- Long Name
- Ribosomal Protein S6 Kinase B1
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3935z | Mouse Anti-RPS6KB1 Recombinant Antibody (clone 30G9) | WB, ELISA, FC, IHC | Mouse IgG1 |
| MOB-0167F | Mouse Anti-RPS6KB1 Recombinant Antibody (clone 1C7) | IHC-P, IF | Mouse IgG |
| ZG-0054U | Rabbit Anti-RPS6KB1 Recombinant Antibody (clone 7D4) | ELISA, IF | Rabbit IgG |
| VS3-FY1284 | Recombinant Rabbit Anti-RPS6KB1 Antibody (clone R01-4B5) | WB, ICC, IF, IP | Rabbit IgG |
| VS3-FY1285 | Recombinant Rabbit Anti-RPS6KB1 Antibody (clone R03-2B2) | WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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