Rabbit Anti-RPS6KB1 (pT421/pS424) Recombinant Antibody (clone R09-3B7)
CAT#: VS3-WK2109
This product is a rabbit recombinant antibody that is specific to phosphorylated RPS6KB1. The target of this antibody is phospho-RPS6KB1 (Thr421/Ser424). The clone R09-3B7 can be used in WB analysis at a dilution of 1/500-1/1000 and IP analysis at a dilution of 1/20.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39442/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human bone marrow
Immunohistochemical staining of human bone marrow shows distinct nuclear positivity in hematopoietic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39442/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3838/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human stomach
Immunohistochemical staining of human stomach shows cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/ihc_selected.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/42029_A_9_8.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/42029_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/42029_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enriched (MCF7)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000108443-RPS6KB1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Thr421/Ser424 of human and rat RPS6KB1
- Species Reactivity
- Human, Rat
- Clone
- R09-3B7
- Applications
- WB, IP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IP: 1/20
Target
- Alternative Names
- Ribosomal Protein S6 Kinase B1; Serine/Threonine-Protein Kinase 14A; Ribosomal Protein S6 Kinase I; EC 2.7.11.1; S6K-Beta-1; P70 S6KA; STK14A; S6K1; Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1; Ribosomal Protein S6 Kinase, 70kD, Polypeptide 1; 70 KDa Ribosomal Protein S6 Kinase 1; Ribosomal Protein S6 Kinase Beta-1; Serine/Threonine Kinase 14 Alpha
- Gene ID
- 6198
- UniProt ID
- P23443
- Long Name
- Ribosomal Protein S6 Kinase B1
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
See other products for "RPS6KB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3935z | Mouse Anti-RPS6KB1 Recombinant Antibody (clone 30G9) | WB, ELISA, FC, IHC | Mouse IgG1 |
| MOB-0167F | Mouse Anti-RPS6KB1 Recombinant Antibody (clone 1C7) | IHC-P, IF | Mouse IgG |
| ZG-0054U | Rabbit Anti-RPS6KB1 Recombinant Antibody (clone 7D4) | ELISA, IF | Rabbit IgG |
| VS3-FY1284 | Recombinant Rabbit Anti-RPS6KB1 Antibody (clone R01-4B5) | WB, ICC, IF, IP | Rabbit IgG |
| VS3-FY1285 | Recombinant Rabbit Anti-RPS6KB1 Antibody (clone R03-2B2) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3126 | Hi-Affi™ Recombinant Rabbit Anti-RPS6KB1 Monoclonal Antibody (DS3126AB) | IHC-P | IgG |
| MOR-4660 | Hi-Affi™ Recombinant Rabbit Anti-RPS6KB1 Monoclonal Antibody (TH174DS) | WB, IF, ICC, FC | IgG |
| MOR-4699 | Hi-Affi™ Recombinant Rabbit Anti-RPS6KB1 Monoclonal Antibody (TH213DS) | WB, IF, ICC, FC | IgG |
| MOR-4700 | Hi-Affi™ Recombinant Rabbit Anti-RPS6KB1 Monoclonal Antibody (TH214DS) | WB, FC, ChIP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1968 | Anti-RPS6KB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6231 | Anti-Mouse RPS6KB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6230 | Anti-Human RPS6KB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6232 | Anti-Rat RPS6KB1 Immunohistochemistry Kit | IHC |
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