Rabbit Anti-RPS6KB1 Recombinant Antibody (clone R07-7V3) (CAT#: VS3-XY3490)
This antibody is a recombinant rabbit antibody binding with RPS6KB1 (Ribosomal protein S6 kinase B1). The antibody recognizes human, mouse and rat RPS6KB1. It has been detected using Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Flow cytometry, Immunocytochemistry or Immunofluorescence.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39442/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39442/if_selected.jpg
Figure 2 IHC staining of human bone marrow
(Immunohistochemical staining of human bone marrow shows distinct nuclear positivity in hematopoietic cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39442/ihc_selected.jpg
(Immunohistochemical staining of human bone marrow shows distinct nuclear positivity in hematopoietic cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39442/ihc_selected.jpg
Figure 3 IHC staining of human cerebellum
(Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3838/ihc_selected.jpg
(Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3838/ihc_selected.jpg
Figure 4 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/ihc_selected.jpg
(Immunohistochemical staining of human stomach shows cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/ihc_selected.jpg
Figure 5 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/42029_A_9_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/42029_A_9_8.jpg
Figure 6 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/42029_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/42029_A_8_5.jpg
Figure 7 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/42029_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/42029_A_6_6.jpg
Figure 8 RNA cell line category: Cell line enriched (MCF7)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000108443-RPS6KB1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000108443-RPS6KB1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat RPS6KB1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R07-7V3
- Applications
- WB, IHC-P, ICC, IF, IP, FC
- Related Disease
- Tuberous Sclerosis and Lymphangioleiomyomatosis
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
FC: 1/50-1/100
Target
- Alternative Names
- S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha
- Gene ID
- 6198
- Long Name
- Ribosomal protein S6 kinase B1
- Cellular Localization
- Mitochondrion outer membrane, Mitochondrion
- Post Translation Modifications
- Phosphorylation at Thr-412 is regulated by mTORC1.
The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism (PubMed:29236692, 18925875, 19085255, 22017876, 23429703).
Activated by phosphorylation at Thr-252 by PDPK1 (PubMed:9445476, 19864428).
Dephosphorylation by PPP1CC at Thr-412 in mitochondrion.
- Protein Refseq
- XP_054172845.1
- Function
- Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression.
Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD.
Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3126 | Hi-Affi™ Recombinant Rabbit Anti-RPS6KB1 Monoclonal Antibody (DS3126AB) | IHC-P | IgG |
| MOR-4660 | Hi-Affi™ Recombinant Rabbit Anti-RPS6KB1 Monoclonal Antibody (TH174DS) | WB, IF, ICC, FC | IgG |
| MOR-4699 | Hi-Affi™ Recombinant Rabbit Anti-RPS6KB1 Monoclonal Antibody (TH213DS) | WB, IF, ICC, FC | IgG |
| MOR-4700 | Hi-Affi™ Recombinant Rabbit Anti-RPS6KB1 Monoclonal Antibody (TH214DS) | WB, FC, ChIP | IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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