Mouse Anti-S100A1 Monoclonal Antibody (VS7-0425-WR810)
CAT#: VS7-0425-WR810
This product is a mouse IgG antibody that binds to human S100A1. The antibody can be used for WB, IHC-P, IF, and ELISA assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm, cytosol & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6462/1662_G4_2_selected.jpg
Normal Tissue
Figure 2 Heart muscle
Cardiomyocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6462/20242_B_4_6.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6462/20242_B_9_5.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6462/20242_A_9_5.jpg
RNA Expression
Figure 5 RNA cell line category: Group enriched (Hep G2, SK-MEL-30)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000160678-S100A1
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Specifications
- Immunogen
- Recombinant human S100A1 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human S100A1
- Species Reactivity
- Human
- Molecule Class
- EF-Hand Calcium-Binding Protein Family
- Applications
- WB, IHC-P, IF, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- S100A1; S100 Alpha; Protein S100-A1; S-100 Protein Subunit Alpha; S100 Calcium-Binding Protein A1; S100 Protein, Alpha Polypeptide; S100; S100 Calcium Binding Protein A1; S-100 Protein Alpha Chain; S100-Alpha
- Gene ID
- 6271
- UniProt ID
- P23297
- Long Name
- S100 Calcium Binding Protein A1
- Cellular Localization
- Cytoplasm; Mitochondrion; Sarcoplasmic reticulum
- Post Translation Modifications
- Glutathionylation; S-nitrosylation
- Protein Refseq
- NP_006262.1
- Function
- Small calcium binding protein that plays important roles in several biological processes such as Ca2+ homeostasis, chondrocyte biology and cardiomyocyte regulation (PubMed:12804600).; In response to an increase in intracellular Ca2+ levels, binds calcium which triggers conformational changes (PubMed:23351007).; These changes allow interactions with specific target proteins and modulate their activity (PubMed:22399290).; Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca2+ cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca2+-ATPase/ATP2A2 and mitochondrial F1-ATPase (PubMed:12804600).; Facilitates diastolic Ca2+ dissociation and myofilament mechanics in order to improve relaxation during diastole (PubMed:11717446).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "S100A1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-1251CQ | Mouse Anti-S100A1 Recombinant Antibody (clone S1-61) | WB, IHC | Mouse IgG1 |
| ZG-0390U | Rabbit Anti-S100A1 Recombinant Antibody (clone Q17-N) | ICC | Rabbit IgG |
| VS3-WK209 | Mouse Anti-S100A1 Recombinant Antibody (clone VS3-WK209) | ELISA, CLIA, RIA, IHC, WB | Mouse IgG |
| VS3-CJ795 | Mouse Anti-S100A1 Recombinant Antibody (clone PT0234) | IHC-P | Mouse IgG1, κ |
| VS3-CJ796 | Mouse Anti-S100A1 Recombinant Antibody (clone PT1427) | IHC-P | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1986 | Anti-S100A1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6293 | Anti-Human S100A1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6294 | Anti-Mouse S100A1 Immunohistochemistry Kit | IHC |
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