Mouse Anti-S100A6 Recombinant Antibody (clone 7D11)
CAT#: VS7-0425-WR812
This product is a mouse IgG1 antibody that binds to human S100A6. The antibody 7D11 can be used for IHC assay.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7575/18_B10_1_selected.jpg
Normal Tissue
Figure 2 Urinary bladder
High expression in urinary bladder
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7575/21633_A_4_5_val_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40549/84815_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2601/6726_A_9_4.jpg
Normal Tissue
Figure 5 Pancreas
Exocrine glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Pancreatic endocrine cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2601/6726_A_3_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2601/6726_A_8_5.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2601/6726_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (OE19, RPTEC TERT1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000197956-S100A6
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Specifications
- Immunogen
- Recombinant human S100A6 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human S100A6
- Species Reactivity
- Human
- Clone
- 7D11
- Molecule Class
- EF-Hand Calcium-Binding Protein Family
- Applications
- IHC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- S100 Calcium Binding Protein A6; Prolactin Receptor-Associated Protein; Growth Factor-Inducible Protein 2A9; Calcyclin; MLN 4; CACY; PRA; S100 Calcium-Binding Protein A6 (Calcyclin)
- Gene ID
- 6277
- UniProt ID
- P06703
- Long Name
- S100 Calcium Binding Protein A6
- Cellular Localization
- Cell membrane; Cytoplasm; Membrane; Nucleus
- Post Translation Modifications
- Acetylation; Phosphoprotein
- Protein Refseq
- NP_055439.1
- Function
- May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes such as the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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