Mouse Anti-S100B Monoclonal Antibody (VS7-0425-WR815)
CAT#: VS7-0425-WR815
This product is a mouse IgG antibody that binds to human S100B. The antibody can be used for WB, IHC-P, IF, and ELISA assays.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line SK-MEL-30
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15768/1844_C4_31_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15768/129_E6_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15768/941_H2_1_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73/163243_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73/163243_A_7_3.jpg
Normal Tissue
Figure 6 Soft tissue
Peripheral nerve
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73/163243_B_1_7.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73/163243_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Group enriched (SK-MEL-30, WM-115)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000160307-S100B
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Specifications
- Immunogen
- Recombinant human S100B fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human S100B
- Species Reactivity
- Human
- Molecule Class
- EF-Hand Calcium-Binding Protein Family
- Applications
- WB, IHC-P, IF, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- S100B; S100 calcium binding protein B; NEF; S100; S100-B; S100beta; protein S100-B; S-100 protein subunit beta; S-100 calcium-binding protein, beta chain; S100 calcium-binding protein, beta (neural)
- Gene ID
- 6285
- UniProt ID
- P04271
- Long Name
- S100 calcium binding protein B
- Cellular Localization
- Cytoplasm; Nucleus; Secreted
- Post Translation Modifications
- Acetylation
- Protein Refseq
- NP_006263.1
- Function
- Small zinc- and- and calcium-binding protein that is highly expressed in astrocytes and constitutes one of the most abundant soluble proteins in brain (PubMed:20950652, PubMed:6487634).; Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer (PubMed:20950652, PubMed:6487634).; Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites (By similarity).; Acts as a neurotrophic factor that promotes astrocytosis and axonal proliferation (By similarity).; Involved in innervation of thermogenic adipose tissue by acting as an adipocyte-derived neurotrophic factor that promotes sympathetic innervation of adipose tissue (By similarity).; Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase (By similarity).; Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity).; Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization (PubMed:20351179).; May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity (PubMed:22399290).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "S100B"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-2226-sdAb | Recombinant Anti-Human S100B VHH Single Domain Antibody | WB, IP, ChiP, Neut, ELISA | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1032 | Recombinant Mouse Anti-Human S100B Antibody Fab Fragment | ELISA | Fab |
| HPAB-1326LY-F(E) | Mouse Anti-S100B Recombinant Antibody (clone 1C8); Fab Fragment | Neut, IHC, ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3160 | Hi-Affi™ Recombinant Rabbit Anti-S100B Monoclonal Antibody (DS3160AB) | EIA, IP, RIA, WB | IgG |
| MOR-4729 | Hi-Affi™ Recombinant Rabbit Anti-S100B Monoclonal Antibody (TH243DS) | WB, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1326LY-S(P) | Mouse Anti-S100B Recombinant Antibody (clone 1C8); scfv Fragment | Neut, IHC, ELISA | Mouse scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1326LY | Mouse Anti-S100B Recombinant Antibody (clone 1C8) | Neut, IHC, ELISA | Mouse IgG |
| MRO-1356-CN | Recombinant Rabbit Anti-S100B Monoclonal Antibody (CBACN-493) | WB, IF, IP, IHC | Rabbit IgG |
| VS3-XY1395 | Mouse Anti-S100B Recombinant Antibody (clone 9A11B9) | ELISA, WB, IHC | Mouse IgG1 |
| VS-0223-WK60 | Rabbit Anti-S100b Recombinant Antibody (VS-0223-WK60) | ICC, IHC, IHC-P | Rabbit IgG |
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