Mouse Anti-S100B Monoclonal Antibody (VS7-0425-WR815) (CAT#: VS7-0425-WR815)

• Immunogen
• Recombinant human S100B fragment expressed in E. Coli.

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Human S100B

• Species Reactivity
• Human

• Molecule Class
• EF-Hand Calcium-Binding Protein Family

• Applications
• WB, IHC-P, IF, ELISA

• Conjugate
• Unconjugated

• Clonality
• Monoclonal

• Purity
• >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid

• Format
• Liquid

• Concentration
• 1 mg/ml

• Preservative
• 0.03% sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• Western Blot: 1:500 - 1:2000
  Immunohistochemistry: 1:200 - 1:1000
  Enzyme-linked Immunosorbent Assay: 1:10000

• Alternative Names
• S100B; S100 calcium binding protein B; NEF; S100; S100-B; S100beta; protein S100-B; S-100 protein subunit beta; S-100 calcium-binding protein, beta chain; S100 calcium-binding protein, beta (neural)

• Gene ID
• 6285

• UniProt ID
• P04271

• Long Name
• S100 calcium binding protein B

• Cellular Localization
• Cytoplasm; Nucleus; Secreted

• Post Translation Modifications
• Acetylation

• Protein Refseq
• NP_006263.1

• Function
• Small zinc- and- and calcium-binding protein that is highly expressed in astrocytes and constitutes one of the most abundant soluble proteins in brain (PubMed:20950652, PubMed:6487634).; Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer (PubMed:20950652, PubMed:6487634).; Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites (By similarity).; Acts as a neurotrophic factor that promotes astrocytosis and axonal proliferation (By similarity).; Involved in innervation of thermogenic adipose tissue by acting as an adipocyte-derived neurotrophic factor that promotes sympathetic innervation of adipose tissue (By similarity).; Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase (By similarity).; Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity).; Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization (PubMed:20351179).; May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity (PubMed:22399290).

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

Secondary Antibody

• 2ND-040CQ
• Goat Anti-Mouse IgG (H&L) Antibody

• 2ND-042CQ
• Rabbit Anti-Mouse IgG (H&L) Antibody

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