AbPlus™ Anti-SAE1 Magnetic Beads (VS-0724-YC1142)
CAT#: VS-0724-YC1142
The AbPlus Anti-SAE1 Magnetic Beads (VS-0724-YC1142) is an innovative affinity resin which is bound with anti-SAE1 specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of SAE1 protein under native and denaturing conditions.
Gene Expression
Normal Tissue
Figure 1 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Nuclear Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41906/89129_B_9_5.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41906/89129_A_8_3.jpg
Normal Tissue
Figure 3 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43552/107661_A_8_4.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41906/89129_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: Medium Intensity: Strong Quantity: <25% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41906/89129_A_4_6.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000142230-SAE1
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Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-SAE1 specific antibody
- Target
- SAE1
- Immunogen
- Recombinant human SAE1, aa 1-346.
- Target Species
- Human
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]
- Alternative Names
- SUMO1 Activating Enzyme Subunit 1; Ubiquitin-Like 1-Activating Enzyme E1A; UBLE1A; AOS1; SUA1; Ubiquitin-Like Protein SUMO-1 Activating Enzyme; SUMO-1 Activating Enzyme E1 N Subunit;
- Gene ID
- 10055
- UniProt ID
- Q9UBE0
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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See other products for "SAE1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3851z | Mouse Anti-SAE1 Recombinant Antibody (clone 42G11) | WB, IP, IF, ELISA | Mouse IgG2a, κ |
| MOB-1454MZ | Recombinant Mouse Anti-Human SAE1 Antibody (clone 1G4-1G5) | IHC-P, WB | Mouse antibody |
| MRO-1361-CN | Recombinant Rabbit Anti-SAE1 Monoclonal Antibody (JG35-88) | WB, FC | Rabbit IgG |
| ZG-0070U | Rabbit Anti-SAE1 Recombinant Antibody (clone 9D8) | ELISA, WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1034 | CytoStream™ Rabbit Anti-SAE1 Recombinant Antibody (VS13-YC1034) | WB, ICC, IF, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6333 | Anti-SAE1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6334 | Anti-Mouse SAE1 Immunohistochemistry Kit | IHC |
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