Rabbit Anti-SAE1 Recombinant Antibody (clone 9D8) (CAT#: ZG-0070U)
The purified Rabbit Anti-SAE1 Recombinant Antibody (clone 9D8) displayed high specificity against the SAE1 protein of human.
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WB
Figure 1 Rabbit Anti-SAE1 Antibody (ZG-0070U) in WB
Positive WB detected in: K562 whole cell lysate. All lanes: SAE1 Antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39, 30, 34 kDa
Observed band size: 39 kDa
IF
Figure 2 Rabbit Anti-SAE1 Antibody (ZG-0070U) in IF
Immunofluorescence staining of Hela Cells with ZG-0070U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human SAE1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SAE1
- Species Reactivity
- Human
- Clone
- 9D8
- Applications
- ELISA, WB, IF
- Conjugate
- Unconjugated
- Related Disease
- Ogden Syndrome and Adult Dermatomyositis
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot, Immunofluorescence. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IF:1:20-1:200
Target
- Alternative Names
- SUMO-activating enzyme subunit 1; Ubiquitin-like 1-activating enzyme E1A; Cleaved into: SUMO-activating enzyme subunit 1; N-terminally processed; SAE1; AOS1; SUA1; UBLE1A
- Gene ID
- 10055
- UniProt ID
- Q9UBE0
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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|---|---|---|---|
| MOB-3851z | Mouse Anti-SAE1 Recombinant Antibody (clone 42G11) | WB, IP, IF, ELISA | Mouse IgG2a, κ |
| MOB-1454MZ | Recombinant Mouse Anti-Human SAE1 Antibody (clone 1G4-1G5) | IHC-P, WB | Mouse antibody |
| MRO-1361-CN | Recombinant Rabbit Anti-SAE1 Monoclonal Antibody (JG35-88) | WB, FC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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