Rabbit Anti-SAE1 Recombinant Antibody (clone 9D8)
CAT#: ZG-0070U
The purified Rabbit Anti-SAE1 Recombinant Antibody (clone 9D8) displayed high specificity against the SAE1 protein of human.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-SAE1 Antibody (ZG-0070U) in WB
Positive WB detected in: K562 whole cell lysate. All lanes: SAE1 Antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39, 30, 34 kDa
Observed band size: 39 kDa
IF
Figure 2 Rabbit Anti-SAE1 Antibody (ZG-0070U) in IF
Immunofluorescence staining of Hela Cells with ZG-0070U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
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Normal Tissue
Figure 1 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Nuclear Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41906/89129_B_9_5.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41906/89129_A_8_3.jpg
Normal Tissue
Figure 3 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43552/107661_A_8_4.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41906/89129_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: Medium Intensity: Strong Quantity: <25% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41906/89129_A_4_6.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000142230-SAE1
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Specifications
- Immunogen
- A synthesized peptide derived from human SAE1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SAE1
- Species Reactivity
- Human
- Clone
- 9D8
- Applications
- ELISA, WB, IF
- Conjugate
- Unconjugated
- Related Disease
- Ogden Syndrome and Adult Dermatomyositis
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot, Immunofluorescence. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IF:1:20-1:200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| MOB-3851z | Mouse Anti-SAE1 Recombinant Antibody (clone 42G11) | WB, IP, IF, ELISA | Mouse IgG2a, κ |
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| MRO-1361-CN | Recombinant Rabbit Anti-SAE1 Monoclonal Antibody (JG35-88) | WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| VS-0724-YC1142 | AbPlus™ Anti-SAE1 Magnetic Beads (VS-0724-YC1142) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1034 | CytoStream™ Rabbit Anti-SAE1 Recombinant Antibody (VS13-YC1034) | WB, ICC, IF, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6333 | Anti-SAE1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6334 | Anti-Mouse SAE1 Immunohistochemistry Kit | IHC |
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