Human Anti-SARS-CoV-2 S protein Recombinant Antibody (clone 10-28) (CAT#: VS-0623-WK36)
The antibody clone 10-28 was produced by immunizing with recombinant SARS-CoV-2 S protein. The antibody recognized epitopes within the receptor binding domain (RBD) and inhibited the binding of soluble human angiotensin converting enzyme 2 (ACE2) receptor to the S protein. It can be used for detection of SARS-CoV-2 S protein in ELISA and SPR assays.
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ELISA
Figure 1 10-28, 10-40, and 11-11 were tested for binding to SARS CoV-2 D614G, B.1.351, SARS-CoV, as well as the RBD of D614G.
The horizontal dashed lines indicate OD=1.2, the half-maximal binding value.
Inhib
Figure 2 Inhibition of ACE2 binding to SARSCoV-2 D614G, B.1.351, and SARS-CoV S proteins was tested for 10-28, 10-40, and 11-11.
Neut
Figure 3 Neutralization curves of selected mAbs against VSV pseudotypes with D614G (WT) and B.1.1.529.1 S proteins are shown.
The dotted horizontal line at 50% indicates IC50 values.
FC
Figure 4 Binding of mAbs to S proteins expressed on the surface of transfected cells was measured by flow cytometry.
Specifications
- Immunogen
- Recombinant SARS-CoV-2 S protein
- Host Species
- Human
- Type
- Human IgG
- Specificity
- SARS-CoV-2 S protein
- Species Reactivity
- SARS-CoV-2 (D614G and B.1.351 variant)
- Clone
- 10-28
- Applications
- ELISA, SPR, Neut, Inhib
- Conjugate
- Unconjugated
- Related Disease
- COVID-19
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No Preservative
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0623-WK35 | Human Anti-SARS-CoV-2 S protein Recombinant Antibody (clone 10-40) | ELISA, SPR, Neut, Inhib, FC, FuncS | Human IgG |
| VS-0623-WK37 | Human Anti-SARS-CoV-2 S protein Recombinant Antibody (clone 11-11) | ELISA, SPR, Neut, Inhib, FC | Human IgG |
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For Research Use Only. Not For Clinical Use.
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