Chicken Anti-SARS-CoV S Recombinant Antibody (clone 4S1)
CAT#: VS-0323-CJ47
This chicken anti-SARS-CoV S antibody (clone 4S1) is a product that recognizes SARS-CoV S proteins.
Published Data
ELISA
Figure 1 Binding activity of randomly chosen phages from library 4S after the fourth panning cycle.
Fifteen phage clones (including VS-0323-CJ47) were amplified and prepared to test their binding to SARS-CoV-infected Vero E6 cell lysates in an ELISA test.
IF
Figure 2 Binding activity of 4S1 phage binder to native S protein.
Commercially available Biochips, precoated with SARS-CoV-infected Vero E6 cells were immunostained with VS-0323-CJ47.
ELISA
Figure 3 Epitope mapped by 4S1 phage binder.
VS-0323-CJ47 was examined for their binding activity to 10 purified and truncated S protein fragments coated on ELISA plates.
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Specifications
- Immunogen
- Recombinant S proteins
- Host Species
- Chicken
- Type
- Chicken IgY
- Specificity
- SARS-CoV S proteins
- Species Reactivity
- SARS-CoV
- Clone
- 4S1
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence
- Conjugate
- Unconjugated
- Related Disease
- Severe acute respiratory syndrome (SARS)
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The Phage clone of this antibody exhibited significant binding activity (OD between 0.7 and 1.2) to SARS-CoV S protein. Phage 4S1 bind to SARS-CoV-infected Vero E6 cells, whose binding activity is comparable to that of convalescent patient serum.
Target
- Alternative Names
- E2
- Gene ID
- 1489668
- UniProt ID
- P59594
- Sequence Similarities
- Belongs to the betacoronaviruses spike protein family.
- Cellular Localization
- Host cell membrane; Host membrane; Membrane; Viral envelope protein; Virion
- Post Translation Modifications
- The cytoplasmic Cys-rich domain is palmitoylated. Spike glycoprotein is digested by cathepsin CTSL within endosomes.
Specific enzymatic cleavages in vivo yield mature proteins. The precursor is processed into S1 and S2 by host cell furin or another cellular protease to yield the mature S1 and S2 proteins. Additionally, a second cleavage leads to the release of a fusion peptide after viral attachment to host cell receptor.
The cytoplasmic Cys-rich domain is palmitoylated. Spike glycoprotein is digested within host endosomes.
- Protein Refseq
- NP_828851.1
- Function
- Spike glycoprotein: May down-regulate host tetherin (BST2) by lysosomal degradation, thereby counteracting its antiviral activity.
Spike protein S1: Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Binding to human ACE2 and CLEC4M/DC-SIGNR receptors and internalization of the virus into the endosomes of the host cell induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membrane fusion within endosomes.
Spike protein S2: Mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
Spike protein S2': Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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