Rabbit Anti-SCAI Recombinant Antibody (clone R05-3J2) (CAT#: VS3-FY1876)
The R05-3J2 recombinant monoclonal antibody targets for human SCAI protein, a calculated weight of 70 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R05-3J2 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:50-1:100), immunofluorescence (dilution range 1:50-1:200).
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20632/222_B10_1_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20632/222_B10_1_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20632/47337_B_8_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20632/47337_B_8_5.jpg
Figure 3 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20632/47337_A_9_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20632/47337_A_9_3.jpg
Figure 4 Kidney
(Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20632/47337_A_9_5.jpg
(Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20632/47337_A_9_5.jpg
Figure 5 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20632/47337_A_4_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20632/47337_A_4_6.jpg
Figure 6 Lymph node
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20632/47337_A_9_8.jpg
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20632/47337_A_9_8.jpg
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000173611-SCAI
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000173611-SCAI
Specifications
- Immunogen
- Synthetic peptides of human SCAI
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat SCAI
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R05-3J2
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 70 kDa, while the observed molecular weight stands at 60 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Suppressor Of Cancer Cell Invasion; Suppressor Of Cancer Cell Invasion Protein; C9orf126; Chromosome 9 Open Reading Frame 126; Protein SCAI; NET40
- Gene ID
- 286205
- UniProt ID
- Q8N9R8
- Sequence Similarities
- Belongs to the SCAI family.
- Cellular Localization
- Cytoplasm, Membrane, Nucleus
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (Q8N9R8)
Modification sites at neXtProt (NX_Q8N9R8)
- Protein Refseq
- NP_001138349.1; NP_775961.2
- Function
- Tumor suppressor which functions to suppress MRTFA-induced SRF transcriptional activity. May function in the RHOA-DIAPH1 signal transduction pathway and regulate cell migration through transcriptional regulation of ITGB1.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "SCAI"
Select a product category from the dropdown menu below to view related products.
Please select product type
Rabbit Monoclonal Antibody Products
IgM Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3177 | Hi-Affi™ Recombinant Rabbit Anti-SCAI Monoclonal Antibody (DS3177AB) | WB, FC | IgG |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for VS3-FY1876. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: ELISA, Inhib, FC
Application: ELISA, FC, WB, FuncS
Application: ELISA, FC, Inhib, FuncS
Application: ELISA, IF, Block, FuncS
-2.png)
Application: ELISA
-4.jpg)
Application: FC
Application: ELISA, FC, Inhib, IHC-Fr, WB, IP
Application: ELISA, WB
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
