CytoStream™ Rabbit Anti-SDHA Recombinant Antibody (VS13-YC1045)
CAT#: VS13-YC1045
The CytoStream™ Rabbit Anti-SDHA Recombinant Antibody is specific for human and mouse SDHA. SDHA encodes a succinate dehydrogenase subunit of the mitochondrial respiratory chain, with mutations associated with Leigh Syndrome and splicing variants. The anti-SDHA antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nucleoli & mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64582/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human stomach, upper
Immunohistochemical staining of human stomach, upper shows strong granular cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/ihc_selected.jpg
Normal Tissue
Figure 4 Testis
Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_6_6.jpg
Normal Tissue
Figure 5 Heart muscle
Cardiomyocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_B_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_7_8.jpg
Normal Tissue
Figure 7 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_8_3.jpg
Normal Tissue
Figure 8 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_8_4.jpg
Normal Tissue
Figure 9 Kidney
Collecting ducts Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_8_5.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000073578-SDHA
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Specifications
- Immunogen
- Recombinant human SDHA, aa 581-624.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse SDHA
- Species Reactivity
- Human, Mouse
- Applications
- WB, ICC, IF, IHC-P, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/5000
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Succinate Dehydrogenase Complex Flavoprotein Subunit A; Flavoprotein Subunit Of Complex II; Succinate Dehydrogenase Complex, Subunit A, Flavoprotein (Fp); SDH2; SDHF; FP; Succinate Dehydrogenase [Ubiquinone] Flavoprotein Subunit, Mitochondrial;
- Gene ID
- 6389
- UniProt ID
- P31040
- Long Name
- Succinate Dehydrogenase Complex Flavoprotein Subunit A
- Cellular Localization
- Cell Membrane; Mitochondrion inner membrane
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "SDHA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1595z | Mouse Anti-SDHA Recombinant Antibody (clone 28G3) | WB, ELISA, IF, IHC | Mouse IgG1 |
| MOB-2460CT | Recombinant Mouse anti-Cow SDHA Monoclonal antibody (7H11.2) | ICC, IHC-Fr, FC, WB, IHC-P | |
| MRO-1372-CN | Recombinant Rabbit Anti-SDHA Monoclonal Antibody (JM10-83) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-FY1325 | Recombinant Rabbit Anti-SDHA Antibody (clone R09-5K6) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
| VS7-0425-WR835 | Mouse Anti-SDHA Recombinant Antibody (clone 2A10F6) | WB, ICC, FC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3192 | Hi-Affi™ Recombinant Rabbit Anti-SDHA Monoclonal Antibody (DS3192AB) | WB, IHC, ICC, IP, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2016 | Anti-SDHA Immunohistochemistry Kit | IHC | |
| VS-0525-XY6411 | Anti-Human SDHA Immunohistochemistry Kit | IHC |
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