CytoStream™ Rabbit Anti-SDHA Recombinant Antibody (VS13-YC1045) (CAT#: VS13-YC1045)
The CytoStream™ Rabbit Anti-SDHA Recombinant Antibody is specific for human and mouse SDHA. SDHA encodes a succinate dehydrogenase subunit of the mitochondrial respiratory chain, with mutations associated with Leigh Syndrome and splicing variants. The anti-SDHA antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/if_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/if_selected.jpg
Figure 2 IF staining of human cell line SiHa
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoli & mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64582/if_selected.jpg
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoli & mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64582/if_selected.jpg
Figure 3 IHC staining of human stomach, upper
(Immunohistochemical staining of human stomach, upper shows strong granular cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/ihc_selected.jpg
(Immunohistochemical staining of human stomach, upper shows strong granular cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/ihc_selected.jpg
Figure 4 Testis
(Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_6_6.jpg
(Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_6_6.jpg
Figure 5 Heart muscle
(Cardiomyocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_B_4_6.jpg
(Cardiomyocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_B_4_6.jpg
Figure 6 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_7_8.jpg
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_7_8.jpg
Figure 7 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_8_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_8_3.jpg
Figure 8 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_8_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_8_4.jpg
Figure 9 Kidney
(Collecting ducts Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_8_5.jpg
(Collecting ducts Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41981/124437_A_8_5.jpg
Figure 10 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000073578-SDHA
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000073578-SDHA
Specifications
- Immunogen
- Recombinant human SDHA, aa 581-624.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse SDHA
- Species Reactivity
- Human, Mouse
- Applications
- WB, ICC, IF, IHC-P, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/5000
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Succinate Dehydrogenase Complex Flavoprotein Subunit A; Flavoprotein Subunit Of Complex II; Succinate Dehydrogenase Complex, Subunit A, Flavoprotein (Fp); SDH2; SDHF; FP; Succinate Dehydrogenase [Ubiquinone] Flavoprotein Subunit, Mitochondrial;
- Gene ID
- 6389
- UniProt ID
- P31040
- Long Name
- Succinate Dehydrogenase Complex Flavoprotein Subunit A
- Cellular Localization
- Cell Membrane; Mitochondrion inner membrane
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1595z | Mouse Anti-SDHA Recombinant Antibody (clone 28G3) | WB, ELISA, IF, IHC | Mouse IgG1 |
| MOB-2460CT | Recombinant Mouse anti-Cow SDHA Monoclonal antibody (7H11.2) | ICC, IHC-Fr, FC, WB, IHC-P | |
| MRO-1372-CN | Recombinant Rabbit Anti-SDHA Monoclonal Antibody (JM10-83) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-FY1325 | Recombinant Rabbit Anti-SDHA Antibody (clone R09-5K6) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
| VS7-0425-WR835 | Mouse Anti-SDHA Recombinant Antibody (clone 2A10F6) | WB, ICC, FC | Mouse IgG2a |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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