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    Mouse Anti-SELL Recombinant Antibody (clone 8C8F3) (CAT#: VS7-0425-WR838)

    This product is a mouse IgG2a antibody that binds to human SELL. The antibody 8C8F3 recognizes specific amino acid domain of human SELL (AA: 83-186). It can be used for IHC and FC assays.

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    • Conjugation:

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    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
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    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line REH
    Figure 1 IF staining of human cell line REH
    (Immunofluorescent staining of human cell line REH shows localization to cytosol.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51972/1676_B10_3_selected.jpg
    Figure 2 IF staining of human cell line MCF7
    Figure 2 IF staining of human cell line MCF7
    (Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51972/1704_H2_7_cr57d82833dfda8_blue_red_green.jpg
    Figure 3 IF staining of human cell line U-2 OS
    Figure 3 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51972/1674_B10_31_blue_red_green.jpg
    Normal Tissue
    Figure 4 Appendix
    Figure 4 Appendix
    (Lymphoid tissue
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2144/5974_A_3_2.jpg
    Figure 5 Lymph node
    Figure 5 Lymph node
    (Germinal center cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous
    Non-germinal center cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2144/5974_A_8_8.jpg
    RNA Expression
    Figure 6 RNA cell line category: Cell line enhanced (Daudi, JURKAT, MOLT-4, U-266/70, U-698, U-937)
    Figure 6 RNA cell line category: Cell line enhanced (Daudi, JURKAT, MOLT-4, U-266/70, U-698, U-937)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000188404-SELL

    Specifications

    • Immunogen
    • Recombinant human SELL (AA: 83-186) fragment expressed in E. Coli.
    • Host Species
    • Mouse
    • Type
    • Mouse IgG2a
    • Specificity
    • Human SELL
    • Species Reactivity
    • Human
    • Clone
    • 8C8F3
    • Molecule Class
    • Cell Surface Adhesion Molecule
    • Applications
    • IHC, FC
    • Conjugate
    • Unconjugated

    Product Property

    • Clonality
    • Monoclonal
    • Purity
    • >95% as determined by SDS-PAGE and SEC-HPLC analysis
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • PBS
    • Preservative
    • 0.05% sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • Western Blot: 1:500 - 1:2000
      Immunohistochemistry-Paraffin: 1:200 - 1:1000
      Flow Cytometry: 1:200 - 1:400
      Enzyme-linked Immunosorbent Assay: 1:10000

    Target

    • Alternative Names
    • TQ1; LAM1; LEU8; LNHR; LSEL; CD62L; LYAM1; PLNHR; LECAM1
    • Long Name
    • Selectin L
    • Cellular Localization
    • Cell membrane; Membrane
    • Post Translation Modifications
    • Disulfide bond; Glycoprotein
    • Function
    • Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells (PubMed:12403782, PubMed:28011641, PubMed:28489325).; Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia (PubMed:12403782, PubMed:28011641).

    Recommended Products

    Isotype Control

    Secondary Antibody

    Reconstitution Buffer

    Recommended Dilution Buffer

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "SELL"

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    Please select product type
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    CAT Product Name Application Type
    NAB-2242-VHH Recombinant Anti-Human SELL VHH Single Domain Antibody Neut, ChiP, IP, FUNC Llama VHH

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