Rabbit Anti-SERPINA1 Recombinant Antibody (clone R01-1G6)
CAT#: VS3-FY1968
By using alpha 1-antitrypsin antibody, western blot analysis of alpha 1 antitrypsin in Hela, A549, MCF-7, U251, and HL-60 lysates was conducted. This incredible rabbit recombinant monoclonal antibody that demonstrated capability to bind to human SERPINA1. It is suitable for use in a variety of assays, including WB, IP.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/927/1399_A10_5_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/927/2684_B_7_5.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16648/40122_A_8_5.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/927/2684_A_6_6.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enriched (Hep G2)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000197249-SERPINA1
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WB
Figure 1 Anti-SERPINA1 Antibody (VS3-FY1968) in WB.
The alpha 1 Antitrypsin expression in Hela, A549, MCF-7, U251, HL-60 lysates were detected using the anti-alpha 1-Antitrypsin by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human α1 antitrypsin
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SERPINA1
- Species Reactivity
- Human
- Clone
- R01-1G6
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 47 kDa, while the observed molecular weight stands at 55 kDa.
- Related Disease
- Emphysema or liver disease
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Serpin Family A Member 1; Serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1; Protease Inhibitor 1 (Anti-Elastase), Alpha-1-Antitrypsin; Alpha-1 Protease Inhibitor; Alpha-1-Antiproteinase; Serpin A1; AAT; PI; Serine (Or Cysteine) Proteinase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1; Serpin Peptidase Inhibitor Clade A (Alpha-1antiproteinase, Antitrypsin) Member 1; Serine (Or Cysteine) Proteinase Inhibitor, Clade A, Member 1; Alpha-1-Antitrypsin Short Transcript Variant 1C4
- Gene ID
- 5265
- UniProt ID
- P01009
- Sequence Similarities
- Belongs to the serpin family.
- Cellular Localization
- Endoplasmic reticulum, Extracellular matrix, Secreted
- Post Translation Modifications
- N-glycosylated.
Differential glycosylation produces a number of isoforms.
N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary.
The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary.
Glycan at Asn-271 is exclusively di-antennary.
Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4.
The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc.
Some antennae are fucosylated, which forms a Lewis-X determinant.
- Protein Refseq
- XP_016876859.1; XP_047287434.1; XP_047287435.1; XP_054184961.1; XP_054184962.1
- Function
- Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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