Rabbit Anti-SERPINA1 Recombinant Antibody (clone R01-1G6) (CAT#: VS3-FY1968)
By using alpha 1-antitrypsin antibody, western blot analysis of alpha 1 antitrypsin in Hela, A549, MCF-7, U251, and HL-60 lysates was conducted. This incredible rabbit recombinant monoclonal antibody that demonstrated capability to bind to human SERPINA1. It is suitable for use in a variety of assays, including WB, IP.
Specific Inquiry
WB
Figure 1 Anti-SERPINA1 Antibody (VS3-FY1968) in WB.
The alpha 1 Antitrypsin expression in Hela, A549, MCF-7, U251, HL-60 lysates were detected using the anti-alpha 1-Antitrypsin by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human α1 antitrypsin
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SERPINA1
- Species Reactivity
- Human
- Clone
- R01-1G6
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 47 kDa, while the observed molecular weight stands at 55 kDa.
- Related Disease
- Emphysema or liver disease
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Serpin Family A Member 1; Serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1; Protease Inhibitor 1 (Anti-Elastase), Alpha-1-Antitrypsin; Alpha-1 Protease Inhibitor; Alpha-1-Antiproteinase; Serpin A1; AAT; PI; Serine (Or Cysteine) Proteinase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1; Serpin Peptidase Inhibitor Clade A (Alpha-1antiproteinase, Antitrypsin) Member 1; Serine (Or Cysteine) Proteinase Inhibitor, Clade A, Member 1; Alpha-1-Antitrypsin Short Transcript Variant 1C4
- Gene ID
- 5265
- UniProt ID
- P01009
- Sequence Similarities
- Belongs to the serpin family.
- Cellular Localization
- Endoplasmic reticulum, Extracellular matrix, Secreted
- Post Translation Modifications
- N-glycosylated.
Differential glycosylation produces a number of isoforms.
N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary.
The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary.
Glycan at Asn-271 is exclusively di-antennary.
Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4.
The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc.
Some antennae are fucosylated, which forms a Lewis-X determinant.
- Protein Refseq
- XP_016876859.1; XP_047287434.1; XP_047287435.1; XP_054184961.1; XP_054184962.1
- Function
- Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0846MZ | Chicken Anti-alpha-1 Antitrypsin Polyclonal IgY | ELISA, WB | Chicken antibody |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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