Mouse Anti-SETD7 Recombinant Antibody (clone 1B3B8)
CAT#: VS7-0425-WR847
This product is a mouse IgG2a antibody that binds to human SETD7. The antibody 1B3B8 recognizes specific amino acid domain of human SETD7 (AA: 107-366). It can be used for WB and FC assays. It cross reacts with rat and monkey.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoli.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58111/1039_D5_2_red_green.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoli rim & mitotic chromosome.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58111/1147_A6_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli rim.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58111/1037_D5_1_red_green.jpg
Normal Tissue
Figure 4 Esophagus
Squamous epithelial cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58111/131814_B_5_1.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58111/131814_A_9_3.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58111/131814_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000145391-SETD7
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Specifications
- Immunogen
- Recombinant human SETD7 (AA: 107-366) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human SETD7
- Species Reactivity
- Human, Rat, Monkey
- Clone
- 1B3B8
- Molecule Class
- Lysine N-Methyltransferase
- Applications
- WB, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- KMT7; SET7; SET9; SET7/9
- Gene ID
- 80854
- UniProt ID
- Q8WTS6
- Long Name
- SET domain containing 7, histone lysine methyltransferase
- Cellular Localization
- Chromosome; Nucleus
- Post Translation Modifications
- Phosphoprotein
- Protein Refseq
- NP_001293128.1
- Function
- Activator; Chromatin regulator; Methyltransferase; Transferase
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
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- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "SETD7"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-525 | Recombinant Human Anti-Human SETD7 Antibody, clone D283 | IF, ELISA, IP-MS | IgG |
| MOB-1875CT | Recombinant Mouse anti-Human SETD7 Monoclonal antibody (t5F6) | ChIP, ELISA, FC, ICC, IF, IHC-G, IHC-P, WB | |
| VS3-XY3502 | Rabbit Anti-SETD7 Recombinant Antibody (clone R05-3B6) | WB, IP | Rabbit IgG |
| VS11-QX128 | Human Anti-SETD7 Recombinant Antibody (clone RAB-C220) | ELISA, IF, IP, ChIP, FC | Human IgG1, κ |
| VS7-0425-WR848 | Mouse Anti-SETD7 Recombinant Antibody (clone 3D6G11) | WB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-526 | Recombinant Human Anti-Human SETD7 Antibody Fab Fragment, clone D284 | IP, IF, ELISA, IP-MS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3235 | Hi-Affi™ Recombinant Rabbit Anti-SETD7 Monoclonal Antibody (DS3235AB) | WB, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY233 | AbPlus™ Anti-SETD7 Magnetic Beads (D283) | IP-MS, Protein Purification | |
| VS-0724-YC906 | AbPlus™ Anti-SETD7 Magnetic Beads (VS-0724-YC906) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6513 | Anti-SETD7 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6514 | Anti-Mouse SETD7 Immunohistochemistry Kit | IHC |
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