Rabbit Anti-SETD7 Recombinant Antibody (clone R05-3B6)
CAT#: VS3-XY3502
The recombinant rabbit antibody reacts with SETD7 (SET domain containing 7, histone lysine methyltransferase), which plays a central role in the transcriptional activation of genes. This antibody specifically reacts with human, mouse and rat SETD7. It has been tested using Western Blot and Immunoprecipitation. The recommended dilutions of Western Blot and Immunoprecipitation is 1/500-1/1000 and 1/20.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoli.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58111/1039_D5_2_red_green.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoli rim & mitotic chromosome.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58111/1147_A6_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli rim.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58111/1037_D5_1_red_green.jpg
Normal Tissue
Figure 4 Esophagus
Squamous epithelial cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58111/131814_B_5_1.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58111/131814_A_9_3.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58111/131814_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000145391-SETD7
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat SETD7
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R05-3B6
- Applications
- WB, IP
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IP: 1/20
Target
- Alternative Names
- KMT7; SET7; SET9; SET7/9
- Gene ID
- 80854
- UniProt ID
- Q8WTS6
- Long Name
- SET domain containing 7, histone lysine methyltransferase
- Cellular Localization
- Nucleus, Chromosome
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (Q8WTS6)
Modification sites at neXtProt (NX_Q8WTS6)
Glycosylation from GlyGen (Q8WTS6) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_054206895.1; XP_016864150.1
- Function
- Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3 (PubMed:11779497, 11850410, 12588998, 12540855, 16141209).
H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed:12588998, 12540855, 16141209).
Plays a central role in the transcriptional activation of genes such as collagenase or insulin (PubMed:16141209, 12588998).
Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription (PubMed:16141209).
Has also methyltransferase activity toward non-histone proteins such as CGAS, p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "SETD7"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-525 | Recombinant Human Anti-Human SETD7 Antibody, clone D283 | IF, ELISA, IP-MS | IgG |
| MOB-1875CT | Recombinant Mouse anti-Human SETD7 Monoclonal antibody (t5F6) | ChIP, ELISA, FC, ICC, IF, IHC-G, IHC-P, WB | |
| VS11-QX128 | Human Anti-SETD7 Recombinant Antibody (clone RAB-C220) | ELISA, IF, IP, ChIP, FC | Human IgG1, κ |
| VS7-0425-WR847 | Mouse Anti-SETD7 Recombinant Antibody (clone 1B3B8) | WB, FC | Mouse IgG2a |
| VS7-0425-WR848 | Mouse Anti-SETD7 Recombinant Antibody (clone 3D6G11) | WB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-526 | Recombinant Human Anti-Human SETD7 Antibody Fab Fragment, clone D284 | IP, IF, ELISA, IP-MS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3235 | Hi-Affi™ Recombinant Rabbit Anti-SETD7 Monoclonal Antibody (DS3235AB) | WB, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY233 | AbPlus™ Anti-SETD7 Magnetic Beads (D283) | IP-MS, Protein Purification | |
| VS-0724-YC906 | AbPlus™ Anti-SETD7 Magnetic Beads (VS-0724-YC906) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6513 | Anti-SETD7 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6514 | Anti-Mouse SETD7 Immunohistochemistry Kit | IHC |
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