Human Anti-SETD7 Recombinant Antibody (clone RAB-C220)
CAT#: VS11-QX128
This product is a recombinant human antibody that recognizes SETD7. This antibody can be used for the following applications: ELISA, IF, IP, IP-MS, ChIP, FC.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoli.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58111/1039_D5_2_red_green.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoli rim & mitotic chromosome.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58111/1147_A6_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli rim.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58111/1037_D5_1_red_green.jpg
Normal Tissue
Figure 4 Esophagus
Squamous epithelial cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58111/131814_B_5_1.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58111/131814_A_9_3.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58111/131814_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000145391-SETD7
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Specifications
- Immunogen
- SETD7 protein
- Host Species
- Human
- Derivation
- Human
- Type
- Human IgG1, κ
- Specificity
- Human SETD7
- Species Reactivity
- Human
- Clone
- RAB-C220
- Applications
- ELISA, IF, IP, ChIP, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- 1 mg/mL (Please refer to the vial label for the specific concentration.)
- Buffer
- 0.2 μm filtered solution in PBS
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipped at 4°C.
Target
- Alternative Names
- KMT7; SET7; SET9; SET7/9
- Gene ID
- 80854
- UniProt ID
- Q8WTS6
- Long Name
- Histone-lysine N-methyltransferase SETD7
- Sequence Similarities
- Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SET7 subfamily.
- Cellular Localization
- Nucleoli, Nucleoli rim, Mitotic chromosome
- Function
- Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "SETD7"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-525 | Recombinant Human Anti-Human SETD7 Antibody, clone D283 | IF, ELISA, IP-MS | IgG |
| MOB-1875CT | Recombinant Mouse anti-Human SETD7 Monoclonal antibody (t5F6) | ChIP, ELISA, FC, ICC, IF, IHC-G, IHC-P, WB | |
| VS3-XY3502 | Rabbit Anti-SETD7 Recombinant Antibody (clone R05-3B6) | WB, IP | Rabbit IgG |
| VS7-0425-WR847 | Mouse Anti-SETD7 Recombinant Antibody (clone 1B3B8) | WB, FC | Mouse IgG2a |
| VS7-0425-WR848 | Mouse Anti-SETD7 Recombinant Antibody (clone 3D6G11) | WB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-526 | Recombinant Human Anti-Human SETD7 Antibody Fab Fragment, clone D284 | IP, IF, ELISA, IP-MS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3235 | Hi-Affi™ Recombinant Rabbit Anti-SETD7 Monoclonal Antibody (DS3235AB) | WB, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY233 | AbPlus™ Anti-SETD7 Magnetic Beads (D283) | IP-MS, Protein Purification | |
| VS-0724-YC906 | AbPlus™ Anti-SETD7 Magnetic Beads (VS-0724-YC906) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6513 | Anti-SETD7 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6514 | Anti-Mouse SETD7 Immunohistochemistry Kit | IHC |
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