Mouse Anti-SIGLEC15 Recombinant Antibody (HPAB-N0241-YC) (CAT#: HPAB-N0241-YC)

Figure 1 Recombinant Mouse Anti-SIGLEC15 Antibody (HPAB-N0241-YC) in SDS-PAGE

SDS-PAGE analysis of HPAB-N0241-YC in non-reduced and β-mercaptoethanol-reduced conditions.

Figure 2 Recombinant Mouse Anti-SIGLEC15 Antibody (HPAB-N0241-YC) in HPLC

The purity of HPAB-N0241-YC was greater than 95% as determined by SEC-HPLC.

Figure 3 Recombinant Anti-SIGLEC15 Antibody (HPAB-N0241-YC) in WB

Western blot analysis of HPAB-N0241-YC was performed by loading Human Siglec-15 / CD33L3 Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-N0241-YC and HRP Goat Anti-Mouse IgG as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reduced antigen (1 μg)

Figure 4 Recombinant Anti-SIGLEC15 Antibody (HPAB-N0241-YC) in DB

Dot Blot analysis of HPAB-N0241-YC was performed by coating with Human Siglec-15 / CD33L3 Protein (His Tag).
HPAB-N0241-YC incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000)

Figure 5 Recombinant Anti-SIGLEC15 Antibody (HPAB-N0241-YC) in ELISA

ELISA analysis of HPAB-N0241-YC was performed by coating with Human Siglec-15 / CD33L3 Protein (His Tag). Then blocked with BSA, and incubated with HPAB-N0241-YC. The HRP-conjugated goat anti-Mouse IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.