Mouse Anti-SIGLEC8 Recombinant Antibody (clone 1A5B9)
CAT#: VS7-0425-WR853
This product is a mouse IgG2b antibody that binds to human SIGLEC8. The antibody 1A5B9 recognizes specific amino acid domain of human SIGLEC8 (AA: extra 17-216). It can be used for WB, IHC, and FC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/29808_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/29808_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/29808_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/29808_A_8_5.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/29808_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enriched (HMC-1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000105366-SIGLEC8
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Specifications
- Immunogen
- Recombinant human SIGLEC8 (AA: extra 17-216) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human SIGLEC8
- Species Reactivity
- Human
- Clone
- 1A5B9
- Molecule Class
- Sialic Acid-Binding Immunoglobulin-Like Lectin
- Applications
- WB, IHC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- SAF2; SIGLEC-8; SIGLEC8L
- Gene ID
- 27181
- UniProt ID
- Q9NYZ4
- Long Name
- Sialic Acid Binding Ig Like Lectin 8
- Cellular Localization
- Membrane
- Post Translation Modifications
- Disulfide bond; Glycoprotein
- Protein Refseq
- NP_001350477.1
- Function
- Putative adhesion molecule that mediates sialic-acid dependent binding to blood cells (PubMed:10625619, PubMed:10856141).; Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface (PubMed:10625619).; Recognizes simultaneously epitopes having a terminal N-acetylneuraminic acid (sialic acid) and an underlying 6-O-sulfated galactose. Preferentially binds to Gal-6-sulfated sialyl-Lewis X glycan epitopes (PubMed:27357658).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "SIGLEC8"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-452CT | Human Anti-SIGLEC8 Recombinant Antibody (TAB-452CT) | WB, ELISA, FC | Humanized antibody |
| TAB-452CT-S(P) | Human Anti-SIGLEC8 Recombinant Antibody; scFv Fragment (TAB-452CT-S(P)) | WB, ELISA, FC | Human scFv |
| TAB-452CT-F(E) | Human Anti-SIGLEC8 Recombinant Antibody; Fab Fragment (TAB-452CT-F(E)) | WB, ELISA, FC | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1378CT | Recombinant Mouse anti-Human SIGLEC8 Monoclonal antibody (EML1447) | WB | |
| HPAB-S0136-YC | Mouse Anti-SIGLEC8 Recombinant Antibody (HPAB-S0136-YC) | ELISA, FC | Mouse IgG2, κ |
| VS3-FY2872 | Mouse Anti-SIGLEC8 Recombinant Antibody (VS3-FY2872) | WB, IHC, FC, ELISA | Mouse IgG |
| VS-0624-FY31 | Human Anti-SIGLEC8 Recombinant Antibody (clone 2C4) | FC | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-S0136-YC-F(E) | Mouse Anti-SIGLEC8 Recombinant Antibody; Fab Fragment (HPAB-S0136-YC-F(E)) | ELISA, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-S0136-YC-S(P) | Mouse Anti-SIGLEC8 Recombinant Antibody; scFv Fragment (HPAB-S0136-YC-S(P)) | ELISA, FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC446 | Recombinant Anti-SIGLEC8 Vesicular Antibody, EV Displayed (VS-0425-YC446) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6586 | Anti-SIGLEC8 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6587 | Anti-Human SIGLEC8 Immunohistochemistry Kit | IHC |
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