Mouse Anti-SIGLEC8 Recombinant Antibody (clone 1A5B9) (CAT#: VS7-0425-WR853)
This product is a mouse IgG2b antibody that binds to human SIGLEC8. The antibody 1A5B9 recognizes specific amino acid domain of human SIGLEC8 (AA: extra 17-216). It can be used for WB, IHC, and FC assays.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/if_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/29808_B_7_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/29808_B_7_5.jpg
Figure 3 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/29808_A_8_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/29808_A_8_3.jpg
Figure 4 Liver
(Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/29808_A_9_4.jpg
(Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/29808_A_9_4.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/29808_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/29808_A_8_5.jpg
Figure 6 Lymph node
(Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/29808_A_9_8.jpg
(Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/29808_A_9_8.jpg
Figure 7 RNA cell line category: Cell line enriched (HMC-1)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000105366-SIGLEC8
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000105366-SIGLEC8
Specifications
- Immunogen
- Recombinant human SIGLEC8 (AA: extra 17-216) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human SIGLEC8
- Species Reactivity
- Human
- Clone
- 1A5B9
- Molecule Class
- Sialic Acid-Binding Immunoglobulin-Like Lectin
- Applications
- WB, IHC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- SAF2; SIGLEC-8; SIGLEC8L
- Gene ID
- 27181
- UniProt ID
- Q9NYZ4
- Long Name
- Sialic Acid Binding Ig Like Lectin 8
- Cellular Localization
- Membrane
- Post Translation Modifications
- Disulfide bond; Glycoprotein
- Protein Refseq
- NP_001350477.1
- Function
- Putative adhesion molecule that mediates sialic-acid dependent binding to blood cells (PubMed:10625619, PubMed:10856141).; Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface (PubMed:10625619).; Recognizes simultaneously epitopes having a terminal N-acetylneuraminic acid (sialic acid) and an underlying 6-O-sulfated galactose. Preferentially binds to Gal-6-sulfated sialyl-Lewis X glycan epitopes (PubMed:27357658).
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "SIGLEC8"
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Humanized Antibody Products
IgG Antibody Products
Fab Antibody Products
ScFv Antibody Products
Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-452CT | Human Anti-SIGLEC8 Recombinant Antibody (TAB-452CT) | WB, ELISA, FC | Humanized antibody |
| TAB-452CT-S(P) | Human Anti-SIGLEC8 Recombinant Antibody; scFv Fragment (TAB-452CT-S(P)) | WB, ELISA, FC | Human scFv |
| TAB-452CT-F(E) | Human Anti-SIGLEC8 Recombinant Antibody; Fab Fragment (TAB-452CT-F(E)) | WB, ELISA, FC | Humanized Fab |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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