Mouse Anti-SIGLEC8 Recombinant Antibody (VS3-FY2872)

CAT#: VS3-FY2872

The anti-SIGLEC8 antibody is a mouse recombinant monoclonal antibody that binds to human SIGLEC8 protein. It cross-reacts with mouse, rat species. The calculated molecular weight of the protein is about 54 kDa. The monoclonal antibody can be used in the area of Eosinophilic Gastritis and Chronic Inducible Urticaria. The antibody can be utilized for detection of SIGLEC8 by Western Blot (1/500-1/2000), Immunohistochemistry (1/50-1/200), Flow cytometry (1/50-1/200) and ELISA (1/1000-1/10000).

Gene Expression
Figure 1 IF staining of human cell line U-251 MG Figure 2 Cerebral cortex Figure 3 Colon Figure 4 Liver Figure 5 Kidney Figure 6 Lymph node Figure 7 RNA cell line category: Cell line enriched (HMC-1)

Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Human, Mouse and Rat SIGLEC8
  • Species Reactivity
  • Human, Mouse, Rat
  • Applications
  • Western Blot, Immunohistochemistry, Flow cytometry, ELISA
  • Conjugate
  • Unconjugated, FITC
  • MW
  • The molecular weight of the target protein is approximately 54 kDa.
  • Related Disease
  • Eosinophilic Gastritis and Chronic Inducible Urticaria

Product Property

  • Clonality
  • Monoclonal Antibody
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/2000
    Immunohistochemistry: 1/50-1/200
    Flow cytometry: 1/50-1/200
    ELISA: 1/1000-1/10000

Target

  • Alternative Names
  • Sialic Acid Binding Ig Like Lectin 8; Sialoadhesin Family Member 2; SIGLEC-8; SAF-2; SAF2; Sialic Acid Binding Ig-Like Lectin 8; Sialic Acid-Binding Ig-Like Lectin 8; SIGLEC8L; CDw329
  • Sequence Similarities
  • Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
  • Cellular Localization
  • Membrane
  • Post Translation Modifications
  • Glycosylation at Asn249, Asn172, and Asn267 (NX_Q9NYZ4 [NX_Q9NYZ4-1])
    Modification sites at PhosphoSitePlus (Q9NYZ4)
    Glycosylation from GlyConnect SIGL8_HUMAN (570)
    Glycosylation from GlyGen (Q9NYZ4) 4 sites, 27 N-linked glycans (1 site)
  • Function
  • Putative adhesion molecule that mediates sialic-acid dependent binding to red blood cells (PubMed:10856141, 10625619).
    Preferentially binds to alpha-2,3-linked sialic acid.
    Also binds to alpha-2,6-linked sialic acid.
    The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface (PubMed:10625619).
    Recognizes simultaneously epitopes having a terminal N-acetylneuraminic acid (sialic acid) and an underlying 6-O-sulfated galactose.
    Preferentially binds to Gal-6-sulfated sialyl-Lewis X glycan epitopes.
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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Secondary Antibody

Isotype Control

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VS-0425-YC446 Recombinant Anti-SIGLEC8 Vesicular Antibody, EV Displayed (VS-0425-YC446) ELISA, FC, Cell-uptake
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