Mouse Anti-SIGLEC8 Recombinant Antibody (VS3-FY2872)
CAT#: VS3-FY2872
The anti-SIGLEC8 antibody is a mouse recombinant monoclonal antibody that binds to human SIGLEC8 protein. It cross-reacts with mouse, rat species. The calculated molecular weight of the protein is about 54 kDa. The monoclonal antibody can be used in the area of Eosinophilic Gastritis and Chronic Inducible Urticaria. The antibody can be utilized for detection of SIGLEC8 by Western Blot (1/500-1/2000), Immunohistochemistry (1/50-1/200), Flow cytometry (1/50-1/200) and ELISA (1/1000-1/10000).







Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, Mouse and Rat SIGLEC8
- Species Reactivity
- Human, Mouse, Rat
- Applications
- Western Blot, Immunohistochemistry, Flow cytometry, ELISA
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 54 kDa.
- Related Disease
- Eosinophilic Gastritis and Chronic Inducible Urticaria
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunohistochemistry: 1/50-1/200
Flow cytometry: 1/50-1/200
ELISA: 1/1000-1/10000
Target
- Alternative Names
- Sialic Acid Binding Ig Like Lectin 8; Sialoadhesin Family Member 2; SIGLEC-8; SAF-2; SAF2; Sialic Acid Binding Ig-Like Lectin 8; Sialic Acid-Binding Ig-Like Lectin 8; SIGLEC8L; CDw329
- Gene ID
- 27181
- UniProt ID
- Q9NYZ4
- Sequence Similarities
- Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
- Cellular Localization
- Membrane
- Post Translation Modifications
- Glycosylation at Asn249, Asn172, and Asn267 (NX_Q9NYZ4 [NX_Q9NYZ4-1])
Modification sites at PhosphoSitePlus (Q9NYZ4)
Glycosylation from GlyConnect SIGL8_HUMAN (570)
Glycosylation from GlyGen (Q9NYZ4) 4 sites, 27 N-linked glycans (1 site)
- Protein Refseq
- XP_054176546.1; NP_001350477.1; NP_055257.2
- Function
- Putative adhesion molecule that mediates sialic-acid dependent binding to red blood cells (PubMed:10856141, 10625619).
Preferentially binds to alpha-2,3-linked sialic acid.
Also binds to alpha-2,6-linked sialic acid.
The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface (PubMed:10625619).
Recognizes simultaneously epitopes having a terminal N-acetylneuraminic acid (sialic acid) and an underlying 6-O-sulfated galactose.
Preferentially binds to Gal-6-sulfated sialyl-Lewis X glycan epitopes.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "SIGLEC8"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-452CT | Human Anti-SIGLEC8 Recombinant Antibody (TAB-452CT) | WB, ELISA, FC | Humanized antibody |
TAB-452CT-S(P) | Human Anti-SIGLEC8 Recombinant Antibody; scFv Fragment (TAB-452CT-S(P)) | WB, ELISA, FC | Human scFv |
TAB-452CT-F(E) | Human Anti-SIGLEC8 Recombinant Antibody; Fab Fragment (TAB-452CT-F(E)) | WB, ELISA, FC | Humanized Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1378CT | Recombinant Mouse anti-Human SIGLEC8 Monoclonal antibody (EML1447) | WB | |
HPAB-S0136-YC | Mouse Anti-SIGLEC8 Recombinant Antibody (HPAB-S0136-YC) | ELISA, FC | Mouse IgG2, κ |
VS-0624-FY31 | Human Anti-SIGLEC8 Recombinant Antibody (clone 2C4) | FC | Human IgG1 |
VS7-0425-WR853 | Mouse Anti-SIGLEC8 Recombinant Antibody (clone 1A5B9) | WB, IHC, FC | Mouse IgG2b |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-S0136-YC-F(E) | Mouse Anti-SIGLEC8 Recombinant Antibody; Fab Fragment (HPAB-S0136-YC-F(E)) | ELISA, FC | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-S0136-YC-S(P) | Mouse Anti-SIGLEC8 Recombinant Antibody; scFv Fragment (HPAB-S0136-YC-S(P)) | ELISA, FC | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
VS-0425-YC446 | Recombinant Anti-SIGLEC8 Vesicular Antibody, EV Displayed (VS-0425-YC446) | ELISA, FC, Cell-uptake |
CAT | Product Name | Application | Type |
---|---|---|---|
VS-0525-XY6586 | Anti-SIGLEC8 Immunohistochemistry Kit | IHC | |
VS-0525-XY6587 | Anti-Human SIGLEC8 Immunohistochemistry Kit | IHC |
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