Mouse Anti-SIGLEC8 Recombinant Antibody (VS3-FY2872) (CAT#: VS3-FY2872)
The anti-SIGLEC8 antibody is a mouse recombinant monoclonal antibody that binds to human SIGLEC8 protein. It cross-reacts with mouse, rat species. The calculated molecular weight of the protein is about 54 kDa. The monoclonal antibody can be used in the area of Eosinophilic Gastritis and Chronic Inducible Urticaria. The antibody can be utilized for detection of SIGLEC8 by Western Blot (1/500-1/2000), Immunohistochemistry (1/50-1/200), Flow cytometry (1/50-1/200) and ELISA (1/1000-1/10000).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/if_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/29808_B_7_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/29808_B_7_5.jpg
Figure 3 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/29808_A_8_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/29808_A_8_3.jpg
Figure 4 Liver
(Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/29808_A_9_4.jpg
(Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/29808_A_9_4.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/29808_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/29808_A_8_5.jpg
Figure 6 Lymph node
(Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/29808_A_9_8.jpg
(Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12556/29808_A_9_8.jpg
Figure 7 RNA cell line category: Cell line enriched (HMC-1)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000105366-SIGLEC8
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000105366-SIGLEC8
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, Mouse and Rat SIGLEC8
- Species Reactivity
- Human, Mouse, Rat
- Applications
- Western Blot, Immunohistochemistry, Flow cytometry, ELISA
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 54 kDa.
- Related Disease
- Eosinophilic Gastritis and Chronic Inducible Urticaria
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunohistochemistry: 1/50-1/200
Flow cytometry: 1/50-1/200
ELISA: 1/1000-1/10000
Target
- Alternative Names
- Sialic Acid Binding Ig Like Lectin 8; Sialoadhesin Family Member 2; SIGLEC-8; SAF-2; SAF2; Sialic Acid Binding Ig-Like Lectin 8; Sialic Acid-Binding Ig-Like Lectin 8; SIGLEC8L; CDw329
- Gene ID
- 27181
- UniProt ID
- Q9NYZ4
- Sequence Similarities
- Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
- Cellular Localization
- Membrane
- Post Translation Modifications
- Glycosylation at Asn249, Asn172, and Asn267 (NX_Q9NYZ4 [NX_Q9NYZ4-1])
Modification sites at PhosphoSitePlus (Q9NYZ4)
Glycosylation from GlyConnect SIGL8_HUMAN (570)
Glycosylation from GlyGen (Q9NYZ4) 4 sites, 27 N-linked glycans (1 site)
- Protein Refseq
- XP_054176546.1; NP_001350477.1; NP_055257.2
- Function
- Putative adhesion molecule that mediates sialic-acid dependent binding to red blood cells (PubMed:10856141, 10625619).
Preferentially binds to alpha-2,3-linked sialic acid.
Also binds to alpha-2,6-linked sialic acid.
The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface (PubMed:10625619).
Recognizes simultaneously epitopes having a terminal N-acetylneuraminic acid (sialic acid) and an underlying 6-O-sulfated galactose.
Preferentially binds to Gal-6-sulfated sialyl-Lewis X glycan epitopes.
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "SIGLEC8"
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Humanized Antibody Products
IgG Antibody Products
Fab Antibody Products
ScFv Antibody Products
Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-452CT | Human Anti-SIGLEC8 Recombinant Antibody (TAB-452CT) | WB, ELISA, FC | Humanized antibody |
| TAB-452CT-S(P) | Human Anti-SIGLEC8 Recombinant Antibody; scFv Fragment (TAB-452CT-S(P)) | WB, ELISA, FC | Human scFv |
| TAB-452CT-F(E) | Human Anti-SIGLEC8 Recombinant Antibody; Fab Fragment (TAB-452CT-F(E)) | WB, ELISA, FC | Humanized Fab |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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