Monkey Anti-SIV p27 Recombinant Antibody (B450) (CAT#: HPAB-0745-YJ)
This product is a recombinant Monkey antibody that recognizes SIV p27.
Specific Inquiry
Neut
Figure 1 Contribution of gp41 truncation to neutralization resistance in infection of macaque cells.
(A) Infection of HSC-F cells in the presence (4 μg/ml) or absence of Fab-B404 is shown. HSC-F cells were inoculated with undiluted SIVmac316 and NS, and 10-fold diluted NS. Viral infection was monitored 4 days post-infection using flow cytometry after intracellular staining by B450 (anti-p27 Fab). (B) Neutralization of SIVmac316 and NS by B404 in HSC-F cells are shown. Percent inhibition was calculated by comparison with the infected culture in the absence of B404.
Kuwata, T., Takaki, K., Enomoto, I., Yoshimura, K., & Matsushita, S. (2013). Increased infectivity in human cells and resistance to antibody-mediated neutralization by truncation of the SIV gp41 cytoplasmic tail. Frontiers in Microbiology, 4, 117.
FuncS
Figure 2 Effect of B404-resistant mutations on the incorporation of Env into virions.
Cell lysates (left panels) and virions (right panels) of SIVmac316 (lane 1), SS (lane 2), SN (lane 3), NS (lane 4), and mock-infected control (lane 5), which were prepared from transfected 293T cells, were analyzed by Western blotting. Viral proteins gp120 (A), gp41 (B), and p27 (C) were detected using MAbs M318T (anti-gp120 V2), B408 (anti-gp41 cluster I), and B450 (anti-p27), respectively.
Kuwata, T., Takaki, K., Enomoto, I., Yoshimura, K., & Matsushita, S. (2013). Increased infectivity in human cells and resistance to antibody-mediated neutralization by truncation of the SIV gp41 cytoplasmic tail. Frontiers in Microbiology, 4, 117.
Specifications
- Host Species
- Monkey
- Derivation
- Phage display library
- Type
- Monkey IgG, κ
- Specificity
- SIV
- Species Reactivity
- SIV
- Clone
- B450
- Applications
- ELISA, Neut, WB, FC, FuncS
- Related Disease
- SIV Infections
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Neutralization, Western Blot, Flow Cytometry, Function Assay. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.
Target
- Alternative Names
- p27; SIV; Simian immunodeficiency virus
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0745-YJ-F(E) | Monkey Anti-SIV p27 Recombinant Antibody (B450); Fab Fragment | ELISA, Neut, WB, FC | Monkey Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0745-YJ-S(P) | Monkey Anti-p27 Recombinant Antibody (B450); scFv Fragment | ELISA, Neut, WB, FC | Monkey scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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