Monkey Anti-SIV p27 Recombinant Antibody (B450)
CAT#: HPAB-0745-YJ
This product is a recombinant Monkey antibody that recognizes SIV p27.
Published Data
Neut
Figure 1 Contribution of gp41 truncation to neutralization resistance in infection of macaque cells.
(A) Infection of HSC-F cells in the presence (4 μg/ml) or absence of Fab-B404 is shown. HSC-F cells were inoculated with undiluted SIVmac316 and NS, and 10-fold diluted NS. Viral infection was monitored 4 days post-infection using flow cytometry after intracellular staining by B450 (anti-p27 Fab). (B) Neutralization of SIVmac316 and NS by B404 in HSC-F cells are shown. Percent inhibition was calculated by comparison with the infected culture in the absence of B404.
Kuwata, T., Takaki, K., Enomoto, I., Yoshimura, K., & Matsushita, S. (2013). Increased infectivity in human cells and resistance to antibody-mediated neutralization by truncation of the SIV gp41 cytoplasmic tail. Frontiers in Microbiology, 4, 117.
FuncS
Figure 2 Effect of B404-resistant mutations on the incorporation of Env into virions.
Cell lysates (left panels) and virions (right panels) of SIVmac316 (lane 1), SS (lane 2), SN (lane 3), NS (lane 4), and mock-infected control (lane 5), which were prepared from transfected 293T cells, were analyzed by Western blotting. Viral proteins gp120 (A), gp41 (B), and p27 (C) were detected using MAbs M318T (anti-gp120 V2), B408 (anti-gp41 cluster I), and B450 (anti-p27), respectively.
Kuwata, T., Takaki, K., Enomoto, I., Yoshimura, K., & Matsushita, S. (2013). Increased infectivity in human cells and resistance to antibody-mediated neutralization by truncation of the SIV gp41 cytoplasmic tail. Frontiers in Microbiology, 4, 117.
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Specifications
- Host Species
- Monkey
- Derivation
- Phage display library
- Type
- Monkey IgG, κ
- Specificity
- SIV
- Species Reactivity
- SIV
- Clone
- B450
- Applications
- ELISA, Neut, WB, FC, FuncS
- Related Disease
- SIV Infections
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Neutralization, Western Blot, Flow Cytometry, Function Assay. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.
Target
- Alternative Names
- p27; SIV; Simian immunodeficiency virus
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Kevin Wu25.Nov,22
Highly Dependable for WB and ELISA
I have been using the Monkey Anti-SIV p27 Recombinant Antibody (B450) for my research on SIV infection mechanisms. The antibody is incredibly specific and has provided clear results consistently in Western Blotting and ELISA assays. Highly recommend for SIV-related research!
James Liu09.Jul,23
Excellent for ELISA
Very effective antibody for detecting SIV p27. We used it in various applications such as ELISA, and it performed well. Although, it took some optimization to get the best results, especially in immunohistochemistry.
Q&As
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What is the primary application for the Monkey Anti-SIV p27 Recombinant Antibody (B450)?
A: The primary application for the Monkey Anti-SIV p27 Recombinant Antibody (B450) is in research related to the Simian Immunodeficiency Virus (SIV). It can be used in various applications such as ELISA, Western Blotting, and Flow Cytometry to detect the p27 protein, which is a core protein of SIV.
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Is the Monkey Anti-SIV p27 Recombinant Antibody (B450) specific only to SIV, or does it cross-react with other proteins?
A: The Monkey Anti-SIV p27 Recombinant Antibody (B450) is intended to be highly specific for the SIV p27 protein. However, to ensure specificity and avoid cross-reactivity, it is always recommended to validate the antibody in your specific experimental setup.
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What is the recommended storage condition for the Monkey Anti-SIV p27 Recombinant Antibody (B450)?
A: For long-term storage, it is advised to store the Monkey Anti-SIV p27 Recombinant Antibody (B450) at -20°C. The antibody can be stored for a brief period of time at 4°C.
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How should the Monkey Anti-SIV p27 Recombinant Antibody (B450) be diluted for use in Western Blotting?
A: The optimal dilution of the Monkey Anti-SIV p27 Recombinant Antibody (B450) for Western Blotting can vary depending on the specific conditions of the experiment. However, a typical starting dilution is 1:1000. It is recommended to perform a titration to determine the optimal dilution for your specific application.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone B450"
- CAT
- Product Name
See other products for "SIV p27"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0745-YJ-F(E) | Monkey Anti-SIV p27 Recombinant Antibody (B450); Fab Fragment | ELISA, Neut, WB, FC | Monkey Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0745-YJ-S(P) | Monkey Anti-p27 Recombinant Antibody (B450); scFv Fragment | ELISA, Neut, WB, FC | Monkey scFv |
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