Rabbit Anti-SLIT2 Recombinant Antibody (clone R51-6S-4) (CAT#: VS3-FY2121)
This R51-6S-4 recombinant monoclonal antibody exhibits high affinity to human SLIT2 protein, a calculated weight of 170 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R51-6S-4 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:100-1:200), immunoprecipitation (dilution range 1:50).
Specific Inquiry
Normal Tissue
RNA Expression
Figure 1 IHC staining of human pancreas
(Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19511/ihc_selected.jpg
(Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19511/ihc_selected.jpg
Figure 2 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23088/ihc_selected.jpg
(Immunohistochemical staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23088/ihc_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23088/51020_B_7_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23088/51020_B_7_5.jpg
Figure 4 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23088/51020_A_7_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23088/51020_A_7_3.jpg
Figure 5 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23088/51020_A_8_4.jpg
(Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23088/51020_A_8_4.jpg
Figure 6 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23088/51020_A_7_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23088/51020_A_7_5.jpg
Figure 7 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23088/51020_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23088/51020_A_6_6.jpg
Figure 8 Placenta
(Decidual cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Trophoblastic cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23088/51020_A_2_7.jpg
(Decidual cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Trophoblastic cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23088/51020_A_2_7.jpg
Figure 9 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+, BJ hTERT+ SV40 Large T+, fHDF/TERT166, HBF TERT88, HEK93)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000145147-SLIT2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000145147-SLIT2
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat SLIT2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R51-6S-4
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 170 kDa, while the observed molecular weight stands at 58kDa.
- Related Disease
- Cakut and Corneal Neovascularization
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Immunoprecipitation: 1/50
Target
- Alternative Names
- Slit Guidance Ligand 2; Slit-2; SLIL3; Slit (Drosophila) Homolog 2; Slit Homolog 2 (Drosophila); Slit Homolog 2 Protein
- Gene ID
- 9353
- UniProt ID
- O94813
- Cellular Localization
- Secreted
- Post Translation Modifications
- Glycosylation at Asn66, Asn186, Asn564, Asn623, Asn794, Asn799, Asn1009, Asn1010, Asn1019, Asn1183, Asn1266, and Asn1300 (NX_O94813 [NX_O94813-1])
Modification sites at PhosphoSitePlus (O94813)
Glycosylation from GlyGen (O94813) 12 sites
- Protein Refseq
- XP_005248268.1; XP_006714049.1; XP_011512211.1; XP_011512212.2; XP_016864334.1
- Function
- Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3318 | Hi-Affi™ Recombinant Rabbit Anti-SLIT2 Monoclonal Antibody (DS3318AB) | IHC-P | IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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