Rabbit Anti-SMAD1 Recombinant Antibody (VS13-YC1076) (CAT#: VS13-YC1076)
The Rabbit Anti-SMAD1 Recombinant Antibody is specific for human and mouse SMAD1. SMAD1 encodes a signaling protein mediating BMP pathways, undergoing phosphorylation and ubiquitination, influencing cellular processes. The anti-SMAD1 antibody can be used for Western blot (1/500), Immunocytochemistry (1/100-1/500), Immunofluorescence (1/100-1/500), Immunohistochemistry-paraffin (1/50-1/200).
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5389/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5389/if_selected.jpg
Figure 2 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows moderate cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5389/19183_A_5_1_selected.jpg
(Immunohistochemical staining of human stomach shows moderate cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5389/19183_A_5_1_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5389/19183_B_7_5.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5389/19183_B_7_5.jpg
Figure 4 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5389/19183_A_9_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5389/19183_A_9_3.jpg
Figure 5 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5389/19183_A_8_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5389/19183_A_8_5.jpg
Figure 6 Testis
(Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5389/19183_A_6_6.jpg
(Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5389/19183_A_6_6.jpg
Figure 7 Lymph node
(Germinal center cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5389/19183_A_9_8.jpg
(Germinal center cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5389/19183_A_9_8.jpg
Figure 8 RNA cell line category: Cell line enhanced (HUVEC TERT2)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000170365-SMAD1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000170365-SMAD1
Specifications
- Immunogen
- Recombinant human Smad1, aa 150-200.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse SMAD1
- Species Reactivity
- Human, Mouse
- Applications
- WB, ICC, IF, IHC-P
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500
Immunocytochemistry (ICC): 1/100-1/500
Immunofluorescence (IF): 1/100-1/500
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Target
- Alternative Names
- SMAD Family Member 1; Mothers Against DPP Homolog 1; Mad-Related Protein 1; MADH1; BSP-1; JV4-1; MADR1; BSP1; MAD, Mothers Against Decapentaplegic Homolog 1 (Drosophila); Transforming Growth Factor-Beta Signaling Protein 1; Transforming Growth Factor-Beta-Signaling Protein 1;
- Gene ID
- 4086
- UniProt ID
- Q15797
- Long Name
- SMAD Family Member 1
- Cellular Localization
- Nucleus; Cytoplasm
- Function
- Signal transduction
Recommended Products
Secondary Antibody
Isotype Control
Related Resources
TGF-β Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibodies
Rabbit Monoclonal Antibodies
IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1884CT | Recombinant Mouse anti-Human SMAD1 Monoclonal antibody (EML536) | ICC, IF, IHC-P, WB | |
| VS3-WK999 | Mouse Anti-SMAD1 Recombinant Antibody (VS3-WK999) | WB | Mouse IgG |
| VS3-WK1000 | Rabbit Anti-SMAD1 Recombinant Antibody (VS3-WK1000) | WB, ICC, IF | Rabbit IgG |
| VS3-FY1355 | Recombinant Mouse Anti-SMAD1 Antibody (clone 3C4G6) | ELISA | Mouse IgG1 |
| VS3-FY1356 | Recombinant Rabbit Anti-SMAD1 (phospho Ser463/Ser465) Antibody (clone R07-4A3) | WB, IP | Rabbit IgG |
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