Mouse Anti-SMAD1 Recombinant Antibody (VS3-CJ87)
CAT#: VS3-CJ87
This product is a mouse antibody that recognizes SMAD1.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5389/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows moderate cytoplasmic and nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5389/19183_A_5_1_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5389/19183_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5389/19183_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5389/19183_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5389/19183_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5389/19183_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (HUVEC TERT2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000170365-SMAD1
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Specifications
- Immunogen
- Recombinant fragment of human SMAD1 (C-terminus)
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human SMAD1
- Species Reactivity
- Human
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- 1 mg/mL (lot specific)
- Buffer
- 50% Glycerol, 1% BSA, PBS, pH7.4.
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in ELISA (1:5000-1:20000), Western Blot (1:100-1:2000).
Target
- Alternative Names
- BSP-1; BSP1; JV4-1; JV41; MADH1; MADR1
- Gene ID
- 4086
- UniProt ID
- Q15797
- Sequence Similarities
- Belongs to the dwarfin/SMAD family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylation of the C-terminal SVS motif by BMP type 1 receptor kinase activates SMAD1 by promoting dissociation from the receptor and trimerization with SMAD4.
Ubiquitinated by SMAD-specific E3 ubiquitin ligase SMURF1, leading to its degradation. Monoubiquitinated, leading to prevent DNA-binding. Deubiquitination by USP15 alleviates inhibition and promotes activation of TGF-beta target genes. Dephosphorylation, probably by PPM1A, induces its export from the nucleus to the cytoplasm (By similarity).
- Protein Refseq
- NP_001003688.1; NP_005891.1
- Function
- Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression.
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Related Signaling Pathways
TGF-β Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
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- Product Name
Secondary Antibody
- CAT
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Recommended Dilution Buffer
- CAT
- Product Name
See other products for "SMAD1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3320 | Hi-Affi™ Recombinant Rabbit Anti-SMAD1 Monoclonal Antibody (DS3320AB) | WB | IgG |
| MOR-4708 | Hi-Affi™ Recombinant Rabbit Anti-SMAD1 Monoclonal Antibody (TH222DS) | WB, IF, ICC, IHC-P, FC | IgG |
| MOR-4734 | Hi-Affi™ Recombinant Rabbit Anti-SMAD1 Monoclonal Antibody (TH248DS) | WB, FC, ChIP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1400-CN | Recombinant Rabbit Anti-SMAD1 Monoclonal Antibody (CBACN-509) | WB, IF | Rabbit IgG |
| MRO-1401-CN | Recombinant Rabbit Anti-SMAD1 Monoclonal Antibody (CBACN-510) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-WK999 | Mouse Anti-SMAD1 Recombinant Antibody (VS3-WK999) | WB | Mouse IgG |
| VS3-WK1000 | Rabbit Anti-SMAD1 Recombinant Antibody (VS3-WK1000) | WB, ICC, IF | Rabbit IgG |
| VS7-0425-WR874 | Mouse Anti-SMAD1 Recombinant Antibody (clone 4E12H10) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2104 | Anti-SMAD1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6714 | Anti-Mouse SMAD1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6713 | Anti-Human SMAD1 Immunohistochemistry Kit | IHC |
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