AbPlus™ Anti-SMAD2 Magnetic Beads (CBACN-511)
CAT#: VS-0424-XY238
The AbPlus Anti-SMAD2 Magnetic Beads (CBACN-511) is an innovative affinity resin which is bound with anti-SMAD2 specific antibody (CBACN-511). The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of SMAD2 protein under native and denaturing conditions.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, nucleoli & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/73546/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in decidual cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/73546/154496_A_3_7_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000175387-SMAD2
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Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magrose bead (> 50 μmol/mL gel)
- Bead Ligand
- Anti-SMAD2 specific antibody (CBACN-511)
- Target
- SMAD2
- Immunogen
- Human SMAD2 (aa 1-200)
- Target Species
- Human, Rat
- Antibody Clone
- CBACN-511
- Bead Capacity
- 20-30 mg/mL binding antibody
- Bead size
- 10-37 μm
- Stability
- pH 2-14
- Format
- 20% Suspension
- Buffer
- PBS, pH 7.4, with 1% BSA
- Preservative
- 0.03% Proclin 300
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene.
- Alternative Names
- SMAD Family Member 2; MAD Homolog 2; HSMAD2; JV18-1; HMAD-2; MADH2; MADR2; MAD, Mothers Against Decapentaplegic Homolog 2 (Drosophila); SMAD, Mothers Against DPP Homolog 2 (Drosophila); Mothers Against Decapentaplegic Homolog 2
- Gene ID
- 4087
- UniProt ID
- Q15796
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Related Diseases
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Related Signaling Pathways
JAK-STAT Signaling Pathway
TGF-β Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4052z | Mouse Anti-SMAD2 Recombinant Antibody (clone 30G7) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
| MRO-2378-CN | Recombinant Rabbit Anti-SMAD2 (phosphorylated Ser250) Monoclonal Antibody (CBACN-664) | WB, FC | Rabbit IgG |
| MRO-2379-CN | Recombinant Rabbit Anti-SMAD2 (phosphorylated Ser255) Monoclonal Antibody (JF0882) | WB, IHC, IP | Rabbit IgG |
| VS3-WK997 | Rabbit Anti-SMAD2 Recombinant Antibody (VS3-WK997) | WB, ICC, IHC, IP, FC | Rabbit IgG |
| VS3-FY1357 | Recombinant Rabbit Anti-SMAD2 (phospho Ser250) Antibody (clone R02-0D1) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3321 | Hi-Affi™ Recombinant Rabbit Anti-SMAD2 Monoclonal Antibody (DS3321AB) | FC, ICC, IHC-P, IP, WB | IgG |
| MOR-4512 | Hi-Affi™ Recombinant Rabbit Anti-SMAD2 Monoclonal Antibody (TH20DS) | WB, IF, ICC | IgG |
| MOR-4709 | Hi-Affi™ Recombinant Rabbit Anti-SMAD2 Monoclonal Antibody (TH223DS) | WB, IF, ICC, FC, ChIP | IgG |
| MOR-4735 | Hi-Affi™ Recombinant Rabbit Anti-SMAD2 Monoclonal Antibody (TH249DS) | WB, IF, ICC, FC, ChIP, ELISA | IgG |
| MOR-0037-FY | Rabbit Anti-SMAD2 Recombinant Antibody (clone AFY0008) | ICC, IHC-P, WB, Inhib | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY484 | Mouse Anti-NHP SMAD2 Recombinant Antibody (VS-1024-XY484) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY71 | Human Anti-SMAD2 scFv-Fc Chimera (VS-0125-FY71) | ADCC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1077 | CytoStream™ Rabbit Anti-SMAD2 Recombinant Antibody (VS13-YC1077) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2105 | Anti-SMAD2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6716 | Anti-Human SMAD2 Immunohistochemistry Kit | IHC |
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