Rabbit Anti-SMAD2 Recombinant Antibody (VS3-FY2873)
CAT#: VS3-FY2873
This anti-SMAD2 antibody is a rabbit recombinant monoclonal antibody that has been intricately crafted to target the human SMAD2 protein. It cross-reacts with rat, hamster species. This monoclonal antibody proves valuable in disease research related to Loeys-Dietz Syndrome 6 and Congenital Heart Defects, Multiple Types, 8, With Or Without Heterotaxy. The antibody can be utilized for detection of target protein by Immunoprecipitation (1/20), Western Blot (1/500-1/1000), Immunohistochemistry (1/50-1/100) and Immunocytochemistry (1/50-1/200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, nucleoli & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/73546/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in decidual cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/73546/154496_A_3_7_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000175387-SMAD2
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Rat and Hamster SMAD2
- Species Reactivity
- Human, Rat, Hamster
- Applications
- Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
- Conjugate
- Unconjugated, FITC
- Related Disease
- Loeys-Dietz Syndrome 6 and Congenital Heart Defects, Multiple Types, 8, With Or Without Heterotaxy
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunoprecipitation: 1/20
Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunocytochemistry: 1/50-1/200
Target
- Alternative Names
- SMAD Family Member 2; MAD Homolog 2; HSMAD2; JV18-1; HMAD-2; MADH2; MADR2; MAD, Mothers Against Decapentaplegic Homolog 2 (Drosophila); SMAD, Mothers Against DPP Homolog 2 (Drosophila); Mothers Against Decapentaplegic Homolog 2
- Gene ID
- 4087
- UniProt ID
- Q15796
- Sequence Similarities
- Belongs to the dwarfin/SMAD family.
- Cellular Localization
- Cytoplasm
Nucleus
- Post Translation Modifications
- Phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255.
In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases.
TGF-beta-induced Ser-465/467 phosphorylation declines progressively in a KMT5A-dependent manner.
Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degradation.
In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2.
Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin.
Phosphorylated by PDPK1.
- Protein Refseq
- XP_047293463.1; XP_047293464.1; XP_054174595.1; XP_054174596.1
- Function
- Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases.
Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription.
Promotes TGFB1-mediated transcription of odontoblastic differentiation genes in dental papilla cells (By similarity).
Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
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Related Diseases
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Related Signaling Pathways
JAK-STAT Signaling Pathway
TGF-β Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "SMAD2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3321 | Hi-Affi™ Recombinant Rabbit Anti-SMAD2 Monoclonal Antibody (DS3321AB) | FC, ICC, IHC-P, IP, WB | IgG |
| MOR-4512 | Hi-Affi™ Recombinant Rabbit Anti-SMAD2 Monoclonal Antibody (TH20DS) | WB, IF, ICC | IgG |
| MOR-4709 | Hi-Affi™ Recombinant Rabbit Anti-SMAD2 Monoclonal Antibody (TH223DS) | WB, IF, ICC, FC, ChIP | IgG |
| MOR-4735 | Hi-Affi™ Recombinant Rabbit Anti-SMAD2 Monoclonal Antibody (TH249DS) | WB, IF, ICC, FC, ChIP, ELISA | IgG |
| MOR-0037-FY | Rabbit Anti-SMAD2 Recombinant Antibody (clone AFY0008) | ICC, IHC-P, WB, Inhib | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1402-CN | Recombinant Mouse Anti-SMAD2 Monoclonal Antibody (C9-B0) | WB, IHC, IF | Mouse IgG1 |
| MRO-1403-CN | Recombinant Rabbit Anti-SMAD2 Monoclonal Antibody (CBACN-511) | WB, IF, IHC, IP, FC | Rabbit IgG |
| MRO-2191-CN | Rabbit Anti-SMAD2 Polyclonal Antibody (MRO-2191-CN) | IF, IHC | Rabbit IgG |
| ZG-093R | Mouse Anti-Smad2 Recombinant Antibody (ZG-093R) | WB, ICC | Mouse IgG |
| ZG-0566U | Rabbit Anti-Phospho-SMAD2 (S250) Recombinant Antibody (clone 4D12) | ELISA, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY238 | AbPlus™ Anti-SMAD2 Magnetic Beads (CBACN-511) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY484 | Mouse Anti-NHP SMAD2 Recombinant Antibody (VS-1024-XY484) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY71 | Human Anti-SMAD2 scFv-Fc Chimera (VS-0125-FY71) | ADCC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1077 | CytoStream™ Rabbit Anti-SMAD2 Recombinant Antibody (VS13-YC1077) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2105 | Anti-SMAD2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6716 | Anti-Human SMAD2 Immunohistochemistry Kit | IHC |
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