Rabbit Anti-SMAD2 Recombinant Antibody (VS3-FY2873) (CAT#: VS3-FY2873)
This anti-SMAD2 antibody is a rabbit recombinant monoclonal antibody that has been intricately crafted to target the human SMAD2 protein. It cross-reacts with rat, hamster species. This monoclonal antibody proves valuable in disease research related to Loeys-Dietz Syndrome 6 and Congenital Heart Defects, Multiple Types, 8, With Or Without Heterotaxy. The antibody can be utilized for detection of target protein by Immunoprecipitation (1/20), Western Blot (1/500-1/1000), Immunohistochemistry (1/50-1/100) and Immunocytochemistry (1/50-1/200).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, nucleoli & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/73546/if_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, nucleoli & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/73546/if_selected.jpg
Figure 2 IHC staining of human placenta
(Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in decidual cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/73546/154496_A_3_7_selected.jpg
(Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in decidual cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/73546/154496_A_3_7_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_B_7_5.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_B_7_5.jpg
Figure 4 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_A_9_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_A_9_3.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_A_7_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_A_7_5.jpg
Figure 6 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_A_4_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_A_4_6.jpg
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000175387-SMAD2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000175387-SMAD2
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Rat and Hamster SMAD2
- Species Reactivity
- Human, Rat, Hamster
- Applications
- Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
- Conjugate
- Unconjugated, FITC
- Related Disease
- Loeys-Dietz Syndrome 6 and Congenital Heart Defects, Multiple Types, 8, With Or Without Heterotaxy
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunoprecipitation: 1/20
Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunocytochemistry: 1/50-1/200
Target
- Alternative Names
- SMAD Family Member 2; MAD Homolog 2; HSMAD2; JV18-1; HMAD-2; MADH2; MADR2; MAD, Mothers Against Decapentaplegic Homolog 2 (Drosophila); SMAD, Mothers Against DPP Homolog 2 (Drosophila); Mothers Against Decapentaplegic Homolog 2
- Gene ID
- 4087
- UniProt ID
- Q15796
- Sequence Similarities
- Belongs to the dwarfin/SMAD family.
- Cellular Localization
- Cytoplasm
Nucleus
- Post Translation Modifications
- Phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255.
In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases.
TGF-beta-induced Ser-465/467 phosphorylation declines progressively in a KMT5A-dependent manner.
Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degradation.
In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2.
Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin.
Phosphorylated by PDPK1.
- Protein Refseq
- XP_047293463.1; XP_047293464.1; XP_054174595.1; XP_054174596.1
- Function
- Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases.
Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription.
Promotes TGFB1-mediated transcription of odontoblastic differentiation genes in dental papilla cells (By similarity).
Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Recommended Products
Secondary Antibody
Isotype Control
Related Resources
Colorectal Cancer
Gastric Cancer
Pancreatic Cancer
AGE-RAGE Signaling Pathway in Diabetic Complications
JAK-STAT Signaling Pathway
TGF-β Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4052z | Mouse Anti-SMAD2 Recombinant Antibody (clone 30G7) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
| MRO-1402-CN | Recombinant Mouse Anti-SMAD2 Monoclonal Antibody (C9-B0) | WB, IHC, IF | Mouse IgG1 |
| MRO-1403-CN | Recombinant Rabbit Anti-SMAD2 Monoclonal Antibody (CBACN-511) | WB, IF, IHC, IP, FC | Rabbit IgG |
| ZG-0566U | Rabbit Anti-Phospho-SMAD2 (S250) Recombinant Antibody (clone 4D12) | ELISA, WB | Rabbit IgG |
| ZG-0567U | Rabbit Anti-Phospho-SMAD2 (S255) Recombinant Antibody (clone 2A12) | ELISA, IF | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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