Rabbit Anti-SMAD2 Recombinant Antibody (VS3-FY2873) (CAT#: VS3-FY2873)

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Rat and Hamster SMAD2

• Species Reactivity
• Human, Rat, Hamster

• Applications
• Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation

• Conjugate
• Unconjugated, FITC

• Related Disease
• Loeys-Dietz Syndrome 6 and Congenital Heart Defects, Multiple Types, 8, With Or Without Heterotaxy

• Clonality
• Monoclonal Antibody

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Immunoprecipitation: 1/20
  Western Blot: 1/500-1/1000
  Immunohistochemistry: 1/50-1/100
  Immunocytochemistry: 1/50-1/200

• Alternative Names
• SMAD Family Member 2; MAD Homolog 2; HSMAD2; JV18-1; HMAD-2; MADH2; MADR2; MAD, Mothers Against Decapentaplegic Homolog 2 (Drosophila); SMAD, Mothers Against DPP Homolog 2 (Drosophila); Mothers Against Decapentaplegic Homolog 2

• Gene ID
• 4087

• UniProt ID
• Q15796

• Sequence Similarities
• Belongs to the dwarfin/SMAD family.

• Cellular Localization
• Cytoplasm
  Nucleus

• Post Translation Modifications
• Phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255.
  In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases.
  TGF-beta-induced Ser-465/467 phosphorylation declines progressively in a KMT5A-dependent manner.
  Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degradation.
  In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2.
  Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin.
  Phosphorylated by PDPK1.

• Protein Refseq
• XP_047293463.1; XP_047293464.1; XP_054174595.1; XP_054174596.1

• Function
• Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases.
  Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription.
  Promotes TGFB1-mediated transcription of odontoblastic differentiation genes in dental papilla cells (By similarity).
  Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

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