Anti-SMAD3 Immunohistochemistry Kit
CAT#: VS-0325-XY2106
This SMAD3 IHC Kit provides a complete set of tools for immunohistochemistry. It is convenient to use as all components are included. It works well with both paraffin and frozen sections. The high - quality antibodies in the kit can precisely detect SMAD3, which is essential for studying the biological functions related to SMAD3, such as its role in mediating TGF - β signaling and extracellular matrix regulation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46386/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8094/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human parathyroid gland
Immunohistochemical staining of human parathyroid gland shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8094/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69409/153288_A_7_2_selected.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69409/153288_A_8_8.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69409/153288_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166949-SMAD3
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Specifications
- Application
- IHC
- Size
- 50 Tests
- Species Reactivity
- Human, Mouse
- Target
- SMAD3
- Primary Antibody
- Rabbit Anti-SMAD3 Antibody
- Secondary Antibody
- Goat anti-Rabbit Antibody, HRP
- Sample Type
- FFPE tissue; Frozen section tissue
- Kit Storage
- All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.
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Related Signaling Pathways
JAK-STAT Signaling Pathway
TGF-β Signaling Pathway
Related Diseases
Pancreatic Cancer
AGE-RAGE Signaling Pathway in Diabetic Complications
Downloadable Resources
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "SMAD3"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3322 | Hi-Affi™ Recombinant Rabbit Anti-SMAD3 Monoclonal Antibody (DS3322AB) | WB, ICC, IHC-P, IP, sELISA | IgG |
| MOR-0052-FY | Rabbit Anti-SMAD3 Recombinant Antibody (clone AFY0023) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1404-CN | Recombinant Rabbit Anti-SMAD3 Monoclonal Antibody (CBACN-512) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-2192-CN | Rabbit Anti-SMAD3 Polyclonal Antibody (MRO-2192-CN) | IF, IHC, FC | Rabbit IgG |
| MRO-2193-CN | Rabbit Anti-SMAD3 Polyclonal Antibody (MRO-2193-CN) | IF, IHC, FC | Rabbit IgG |
| VS3-QX1021 | Mouse Anti-SMAD3 Recombinant Antibody (clone 2A1-8F2-8B8) | IHC | Mouse IgG1 |
| VS3-XY1434 | Mouse Anti-SMAD3 Recombinant Antibody (clone 5G11) | ELISA, WB, ICC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1125 | AbPlus™ Anti-SMAD3 Magnetic Beads (VS-0724-YC1125) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY485 | Rabbit Anti-NHP SMAD3 Recombinant Antibody (clone E.980.9) | WB, IF, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY199 | Human Anti-SMAD3 scFv-Fc Chimera (VS-0325-FY199) | ELISA, IF | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1078 | CytoStream™ Rabbit Anti-SMAD3 Recombinant Antibody (VS13-YC1078) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6718 | Anti-Rat SMAD3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6719 | Anti-Mouse SMAD3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6717 | Anti-Human SMAD3 Immunohistochemistry Kit | IHC |
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